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Ultimately the sole cause of different species is restricted breeding. Males and females of one species will only mate with males and females of the same species.

Now there may be a secondary factor that is the main cause, such as geographic isolation. The two species develop separately because there is a mountain range in between them and the males of each type can't get access to the females of the other type. But as you can see it is the restricted breeding that ultimately does it.

Now for two species of stickleback in the same lake. It may be that the females are finicky in their chose of mates and prefer male with a certain colour pattern, or they prefer nests built at a certain depth which males have an inclination to build at different heights.

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Q: What caused the development of two species of stickleback in the same lake?
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