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The yolk begins to develop in the ovary and after 10-12 days of development, ovulation occurs.

The true egg, the part that develops into the embryo, is located on the egg yolk. It is barely visible to the human eye.

If the Hen has mated with a rooster, sperm will be present and fertilization can occur. The male chicken stand in the hens back to mate. He does not have a penis and therefore there is no penetration of the female reproductive tract at the time of mating. Instead the female inverts the cloaca (the common junction of the reproductive and digestive systems) which comes in contact with the male's inverted cloaca and receives the sperm. The cloaca is then drawn back into the hen's body and the sperm are captured. This is the beginning of the sperms journey up the length of the reproductive system. The sperm live inside the female reproductive system and each time an ovulation occurs (every 24-26 hours in good egg producers) the egg can become fertile. This process continues and matings at 7 - 10 day intervals are necessary to maintain fertility. After fertilization occurs the egg albumen, shell membranes, and shell are added to complete the egg formation process. The chicken usually lays the egg about 24 hours after ovulation.

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