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why RT was shifting & how to RT calculation in HPLC

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Retention time in High Performance Liquid Chromatography (HPLC) refers to the time it takes for a compound to travel through the chromatography column and elute from the detector. It is a key parameter for identifying and characterizing compounds in a sample. Retention time is influenced by factors such as the column type, mobile phase composition, and compound properties.

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People say that I have a retentive memory because I can recall a lot of information.

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