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I imagine it would depend on where exactly they bite you, but generally no, their bite does not hurt. I have lived in Florida my entire life and have caught these lizards and hung them from my ears and my nose. I would just put their mouth near my ear and once they bite down they don't let go. So they will just hang there.

The larger brown anole can pinch a little when you try to pry them back off of you, but that is only because they are a little more aggressive and won't let go.

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Q: Does it hurt when an anole bites?
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