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Assuming there actually is a nucleus in the cell (it isn't undergoing cell division or anything), the nucleus is actually transparent and you see it with dyes that target it. You might be able to see it if it isn't transcriptionally active, when the DNA is condensed inside it.
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TaigaIf a nucleus is not visible through a microscope, it might be due to several reasons, such as its size being below the resolution limit of the microscope, its transparency, or the sample not being stained appropriately to make it visible. Additionally, if the nucleus is not in the field of view or if the microscope is not focused correctly, it may also be difficult to observe.
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Why in some cases nothing could be seen through the microscope?
In some cases, nothing can be seen through a microscope if the sample is too thick or dense for light to pass through effectively. Additionally, if the sample is not mounted properly or if the microscope is not focused correctly, it can also result in not being able to see anything. Proper sample preparation and microscope settings are important for clear visualization.
Describe how a light microscope creates a magnified image?
A light microscope uses lenses to bend and focus light rays passing through a specimen, magnifying the image. The specimen interacts with the light passing through it, which is then transmitted through a series of lenses to produce a magnified image that can be viewed through the eyepiece.
Do large proteins containing a nuclear localization signal bind to the nuclear pore and enter the nucleus without any expenditure of energy?
No, proteins with a nuclear localization signal require energy to actively transport through the nuclear pore complex into the nucleus. They are not able to enter the nucleus passively. The binding and translocation through the nuclear pore are facilitated by specific transport receptors and require energy in the form of GTP hydrolysis.
What happens to light intensity when you move the diaphragm under the stage in a microscope?
As you close the diaphragm by moving it under the stage of a microscope, less light is able to pass through the specimen. This results in a decrease in light intensity, making the image appear dimmer. Adjusting the diaphragm helps control the contrast and brightness of the image.
Why a specimen need to be viewed under the microscope must be thin?
Specimens need to be thin to allow light to pass through them and be able to observe details and structures at a cellular or subcellular level. Thicker specimens would block the light and hinder the ability to visualize the specimen clearly under a microscope. A thin specimen also helps to reduce scattering and distortion of the image.
