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CoachA phase-contrast microscope enhances contrast in transparent specimens by detecting differences in refractive index, making them more visible without the need for staining. In contrast, a bright field microscope relies on the absorption and reflection of light by the sample, requiring staining to enhance contrast.
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In general due to the laws of physics as magnification increases in a microscope the field of view?
As magnification increases in a microscope, the field of view decreases. This is because at higher magnifications, the microscope is focusing on a smaller area of the specimen, resulting in a narrower field of view.
What do you use to measure the field of view in a microscope?
The field of view in a microscope is typically measured using a ruler or a stage micrometer slide that has a scale etched onto it. By calibrating the microscope with these reference tools, you can determine the size of the field of view at different magnifications.
What is To use a ruler to measure the field of view of a microscope called?
This process is called calculating the field of view diameter on a microscope. It involves measuring the diameter of the field of view using a ruler and knowing the magnification of the objective lens to determine the actual size of objects viewed under the microscope.
What is the difference between a microscope's magnification and field of view?
Magnification refers to how much larger an object appears under the microscope compared to the naked eye, while field of view is the diameter of the area visible through the microscope lens at a given magnification. In simple terms, magnification is how big, and field of view is how much you can see.
What does it mean for a microscope to be parcentered?
When a microscope is parcentered, the specimens will appear centered in the field of view at every magnification. So if a field of a slide is centered at the lowest power, even though the field diameter shrinks at each higher magnification, the desired part of the specimen will remain in the center of the viewing field.
