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To investigate absorbance versus time, you would typically measure the absorbance of a sample at regular time intervals using a spectrophotometer. This data can then be plotted on a graph to observe any changes or trends in absorbance over time, which can provide insights into the reaction kinetics or other time-dependent processes happening in the sample. It is important to ensure that the measurement conditions (wavelength, path length, temperature, etc.) remain constant throughout the experiment for accurate results.
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What other experiments would a spectophotometer would be useful?
A spectrophotometer would be useful in experiments involving determining the concentration of a solution by measuring its absorbance, studying the kinetics of enzyme reactions by monitoring changes in absorbance over time, and identifying unknown substances by comparing their absorbance spectra to known compounds.
Does the slope of a speed-versus-time graph represents acceleration?
No, the slope of a speed-versus-time graph represents the rate of change of speed, not acceleration. Acceleration is represented by the slope of a velocity-versus-time graph.
What is the difference between absorbance and transmittance?
Absorbance measures the amount of light absorbed by a sample, while transmittance measures the amount of light that passes through a sample. Absorbance is calculated as -log(T), where T is transmittance. Absorbance is commonly used in spectrophotometry to quantify the concentration of a substance in a solution.
Why do you use the wavelength with the maximum absorbance in spectroscop?
The wavelength with the maximum absorbance corresponds to the peak absorption of the compound being analyzed, providing the most accurate and precise measurement. By measuring absorbance at the maximum wavelength, we can ensure the highest sensitivity and specificity in detecting and quantifying the compound of interest.
Why you use wavelength of 680nm to find absorbance of glucose?
Glucose absorbs light at a specific wavelength of 680nm due to its chemical structure. By measuring the absorbance of glucose at 680nm, we can quantitatively determine the concentration of glucose in a sample through the Beer-Lambert Law, which relates absorbance to concentration.
Is there a simple equation to calculate the concentration given the absorbance and a time?
You need a graphic concentration versus absorbance.
How can you investigate absorbance versus time and not concentration versus time like you normally do for any kinetic investigation?
Too many words
Is wavelength or absorbance the dependent variable?
"absorbance"Since in the experiment, you probably choose the wavelength, then measure the absorbance (absorption?, the absorbance is the dependent variable.
What is the purpose of the blank?
Blank Sample in Spectrophotometry is used to measure the absorbance of light without sample. It is subtracted from the total absorbance for measurement of Absorbance from a sample's absorbance.
What is difference between specific absorbance and absorbance interms of spectroscopy?
specific absorbance- it is absorbance in a solution containing one gm of substance in 100 ml solvent in 1cm shell. so it is having a difference with absorbance which is negative logarithm of incident light to the transmitted light. divya.chakraborty@gmail.com
What is the reason for taking absorbance on y-axis and time on x-axis?
Convention holds that time be on the horizontal axis.
What other experiments would a spectophotometer would be useful?
A spectrophotometer would be useful in experiments involving determining the concentration of a solution by measuring its absorbance, studying the kinetics of enzyme reactions by monitoring changes in absorbance over time, and identifying unknown substances by comparing their absorbance spectra to known compounds.
What is the different between primary absorbance and secondary absorbance in fluorescence?
in primary light absorbed by outer molecule while in secondary re-absorbance occurs
What is the specific absorbance of aspirin?
The specific absorbance of a substance like aspirin refers to its unique ability to absorb light at a specific wavelength. To find the specific absorbance of aspirin, you would need to measure its absorbance at a specific wavelength using a spectrophotometer.
What is baseline in uv?
In UV spectroscopy, the baseline refers to the horizontal line at zero absorbance on the absorbance axis. It represents the reference point for measuring the absorbance of the sample. The baseline should be stable and noise-free to ensure accurate measurement of the absorbance of the sample.
What is the abbreviation for Absorbance?
A
Why are the absorbance values obtained from the conversion chart rather than the absorbance scale on the spectronic 20?
because that chart gives a more accurate value than the absorbance scale on the specthometor
