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Franz Ziehl(Bacteriologist) andFrederick Neelson(pathologist) are the two scientists who discovered Ziehl-Neelson stain or acid-fast stain.Mycobacterium tuberculosis is an organism with high lipid conent in its cell wall because of which acid fast stain is used. 1.smearpreparation 2.flood with cabolfuchsin under continuous heating 3.wash with acid alcohol(20%hci:ethanol) 4.flood methelene blue.wash with water observe under microscope
Ziehl-Neelsen staining is primarily used for the detection of acid-fast bacilli, including Mycobacterium tuberculosis, the causative agent of tuberculosis. The staining procedure involves using a red dye called carbol fuchsin, which helps identify the presence of these bacteria based on their ability to retain the dye despite washing with acid-alcohol. Additionally, Ziehl-Neelsen staining is commonly employed in diagnosing other mycobacterial infections besides tuberculosis, such as leprosy.
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Can dyes or other methylene blue be used for direct staining?
Yes, methylene blue can be used for direct staining in various laboratory techniques such as Gram staining, as it binds to negatively charged molecules in the cell. Other dyes like crystal violet and safranin are commonly used in staining procedures as well.
What is the minimum amount of protein that can appear in SDS PAGE gel?
The minimum amount of protein that can be detected in a SDS-PAGE gel generally depends on the sensitivity of the staining or detection method used. However, in general, amounts as low as 1-10 ng of protein can be detected on an SDS-PAGE gel.
What is staining rack?
A staining rack is a laboratory tool used to hold and organize slides during the staining process. It typically has grooves or slots where slides can be securely placed to prevent them from moving or touching each other while applying different staining solutions. Staining racks help in efficient and uniform staining of multiple slides at once.
Why is safrinin used in gram staining?
as a couterstain
Is carbol fuchsin the same as safranin?
No. safranin is the classic stain used in gram staining. Concentrated Carbol Fushin is mainly used for the ZN staining procedure to stain organisms such as Vibrio cholerae and Cryptosporidium. Diluted Carbol Fushin can however be used as a replacement counterstain for Safranin in the gram stain.
What is the use of coplin jar?
A Coplin jar is used in laboratory settings to hold and process multiple microscope slides at the same time. It is commonly used for staining procedures, such as the Gram stain, where multiple slides need to be immersed in various staining reagents simultaneously.
Used for error detection?
Used for error detection
Can dyes or other methylene blue be used for direct staining?
Yes, methylene blue can be used for direct staining in various laboratory techniques such as Gram staining, as it binds to negatively charged molecules in the cell. Other dyes like crystal violet and safranin are commonly used in staining procedures as well.
What can be used for a simple stain procedure?
Crystal violet, methylene blue, or safranin are commonly used for simple staining procedures. These dyes bind to bacterial cells and color them for visualization under a light microscope.
Uses of staining rack?
used to hold the glass glides while staining them. (:
What is the purpose of differential staining?
differential staining is a staining technique used to stain colorless bacteria against a dark background.
What is the minimum amount of protein that can appear in SDS PAGE gel?
The minimum amount of protein that can be detected in a SDS-PAGE gel generally depends on the sensitivity of the staining or detection method used. However, in general, amounts as low as 1-10 ng of protein can be detected on an SDS-PAGE gel.
What is the function of staining jar?
There are several uses for a staining jar. In microscopy, it is used for staining tissues and cells for slides. After being stained with dyes or stains, the specimens can also be placed in the jar to look for certain aspects.
What is staining rack?
A staining rack is a laboratory tool used to hold and organize slides during the staining process. It typically has grooves or slots where slides can be securely placed to prevent them from moving or touching each other while applying different staining solutions. Staining racks help in efficient and uniform staining of multiple slides at once.
Is endospore staining differencial staining?
Yes, endospore staining is a type of differential staining. It is used to distinguish between bacterial endospores and the vegetative cells of the organism. The endospores appear as green structures against a pink or red background when using the Schaeffer-Fulton staining technique.
Why is safrinin used in gram staining?
as a couterstain
What is the difference between leishman staining and giemsa staining?
Leishman staining is a Romanowsky stain used for identifying parasites like Leishmania, while Giemsa staining is another type of Romanowsky stain used for detecting blood parasites like malaria. Both stains involve a series of dyes that help differentiate the components of cells for microscopic examination.
