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Describe the aseptic technique that would be used when flooding the agar plate with bacteria?
To flood an agar plate with bacteria aseptically, first flame the mouth of the tube containing the bacterial culture. Then, use a sterile pipette to transfer the bacteria onto the agar surface by spreading it evenly in a zig-zag pattern to cover the plate completely. Finally, ensure the plate is properly labeled and incubate it at the appropriate temperature for growth.
What happens when tap water is treated with alum?
the dirts and bacteria in the water would coagolate or clump together~it is also a method of purifying water..
How do you grow bacteria in broth and argar?
To grow bacteria in broth, you would add the bacteria to a sterile liquid broth, incubate it at the optimal temperature for growth, and periodically check for bacterial growth by observing turbidity or colony formation. To grow bacteria on agar, you would spread the bacteria on a sterile agar plate using a spreader, incubate it at the optimal temperature, and observe colony formation.
Why would bacterial colonies found in the first section of a streak plate but not on sections two and three?
If bacterial colonies are found only in the first section of a streak plate, it could be due to uneven streaking technique where the majority of the bacteria were deposited in the initial section. The subsequent sections may not have received enough bacterial cells to form visible colonies. It is important to ensure an even distribution of bacteria while streaking to obtain colonies throughout the plate.
What would you need to do to determine whether bacteria growing on a petri plate from the brewer jar are anaerobes?
You would need to prepare a duplicate set of petri plates, one of which must be incubated in an anaerobic environment using a gas pack or anaerobic jar. If the bacteria only grow on the plate in the anaerobic environment, they are likely anaerobes. Alternatively, you could use anaerobic culture techniques such as thioglycolate broth to confirm the growth characteristics of the bacteria in the absence of oxygen.
How would you change the bacterias plate to best tell if they are ampicillin resistant?
The best test would be to take some of the bacteria growing on the LB plate and streak them on a LB/amp plate. If the bacteria are viable on the LB/amp plate, then they are resistant to ampicillin. If no bacterial colonies survive, then they were not ampicillin resistant.
How Cultivation of bacteria?
To cultivate bacteria, you would typically streak a sample onto a nutrient agar plate in a sterile environment. The plate is then incubated at the optimal temperature for the specific bacteria species to grow. After incubation, colonies of bacteria will form, which can be studied and analyzed.
What would you need to do to determine whether bacteria growing on a petri plate are anaerobes?
some sense
What one plate would you first inspect to conclude that the transformation occurred successfully Why?
I would first inspect the plate that received the highest dilution of the transformed cells, as it would likely have a visible lawn of bacterial colonies if the transformation was successful. This plate is important as it indicates whether there was successful uptake and expression of the foreign DNA by the bacterial cells.
Why do horses stables need disinfecting?
Bacteria or viruses that could be rubbed off. Hand, foot and mouth disease for example. Really horrible I would know from having it personally, so stables and everything have to be kept disinfected.
Describe the aseptic technique that would be used when flooding the agar plate with bacteria?
To flood an agar plate with bacteria aseptically, first flame the mouth of the tube containing the bacterial culture. Then, use a sterile pipette to transfer the bacteria onto the agar surface by spreading it evenly in a zig-zag pattern to cover the plate completely. Finally, ensure the plate is properly labeled and incubate it at the appropriate temperature for growth.
What type of cardiovascular diseases may be treated with antibiotics?
Pericarditis, Endocarditis, and Myocarditis. All of these are inflammation diseases of the heart and if it is caused by bacteria, then it can be treated by antibiotics, because antibiotics clear up infections/bacteria.
What does hydrogen peroxide do for a horse wound?
Don't take me for certain here but as i remember hydrogen peroxide is used for healing and disinfecting. keeping a wound clean from bacteria and etc. So my best guess would be that it would keep your horse's wound disinfected and help it heal up better.
Can fish be given as solid food to 7 month old baby?
No because it would be to big for his or her mouth and it would have to be disinfected
What happens when tap water is treated with alum?
the dirts and bacteria in the water would coagolate or clump together~it is also a method of purifying water..
On which plate would you expect to find bacteria most like the original non transformed E. coli colonies you initially observed?
You would expect to find it in the plate labeled LB-, because that specific plate is the control plate, meaning it has nothing added to it, like the amipicilin or pFlouroGreen plasmid.
Advantages of pour plate method over other methods of bacterial colony?
The purpose of the spread-plate technique is to grow and isolate colonies of bacteria. A sample of bacteria is transferred to the agar plate, an environment that provides nourishment for the bacteria to grow. The bacteria sample is applied to the agar plate which a special streaking technique that dilutes the amount of bacteria in each section of the agar plate continuously. This is because if you just swabbed the bacteria onto the plate with no special technique the colonies would grow very densely together and be difficult to study. The streaking technique gradually dilutes the amount of bacteria in each 'quadrant' of the plate, so the last quadrant should have small, isolated colonies that can be easily studied. The spread plate technique is also used for the eneumeration of aerobic microorganisms from the given sample. This can be done by serial diluting the samples, placing 0.1ml of the diluted sample in the middle of an agar plate and spreading the sample over the surface with a help of an L-rod. After the incubation rhe colonies can be counted.
