Leaving a stain on a slide for too long can lead to the over-staining of the sample, making it difficult to differentiate between different structures or cells. This can result in a loss of contrast and clarity in the sample, affecting the quality of the observation. Additionally, prolonged exposure to the stain can lead to fading or degradation of the sample over time.
Endospores are impermeable to most stains so heat is usually applied to drive the stain into the endospore.
The stain would stain the cells rather than the background
If the slide was heated too hot during heat-fixing, the bacteria on the slide may appear distorted or shriveled. This can make it difficult to accurately identify and classify the bacteria present on the slide. Overheating can also lead to loss of cellular structures and affect the overall staining quality, making it harder to observe the typical gram stain results.
To stain rawhide, first clean and wet the rawhide, then apply leather dye or oil-based stain using a sponge or cloth. Allow the rawhide to dry completely before applying a sealant or finish to protect the stain and the rawhide.
If a bacterial smear is not heat fixed prior to staining, the bacteria may not adhere well to the slide and can wash away during the staining process. Heat fixing helps to kill the bacteria, firmly attach them to the slide, and improve the uptake of stain, resulting in better staining results. Without heat fixing, the bacteria may not stain properly or may not be visible at all under the microscope.
All you have to do is get a yellow stain and just drop one drop and it should spread throughout the water and should turn that color.
The GE CGS980 should neither stain nor tarnish, but if you neglect it and don't clean then it could start to show wear.
The primary stain is selective - it will only highlight certain structures or proteins. The counter stain gives color and definition to the rest of the slide so that the relationship between the highlighted structure and the rest of the cell can be determined.
A smear specimen should be fixed by air-drying the sample or by heat-fixing the slide. This helps to preserve the cellular morphology and prevent degradation of the sample during staining and observation under a microscope. Fixation also improves the adherence of the cells to the slide, making it easier to analyze the sample.
The stain on a wet mount slide helps to enhance the visibility of structures or organisms present on the slide by adding contrast. This allows for easier observation and identification of the specimens under a microscope.
Quickly wet the stain the put some dish washing liquid on it. Rinse out in water after 30 minutes. Allow to dry in the sun. If stain is still visible after drying try vinegar on the stain, dry in sun again, and then wash. Stain should be gone.
Endospores are impermeable to most stains so heat is usually applied to drive the stain into the endospore.
Yes, but with any wood surface that you stain you must have a clean surface. If you have removed all the old paint and you are down to bare wood then you probably should brush on Pre-Stain Wood Conditioner, Minwax runs about 10-11 bucks a quart. If ANY paint or primer is left on wood you will get spots that will only show up after you stain so make sure it is clean and bare.
The stain would stain the cells rather than the background
For a seamless finish, apply the stain before assembling the furniture.
No, Pergo is basically a piece of plastic with a picture of wood on it so if you try to stain it then the graining won't remain plus the stain can damage the protective layer over the image.
If the slide was heated too hot during heat-fixing, the bacteria on the slide may appear distorted or shriveled. This can make it difficult to accurately identify and classify the bacteria present on the slide. Overheating can also lead to loss of cellular structures and affect the overall staining quality, making it harder to observe the typical gram stain results.