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Pressure is important in HPLC to maintain the flow rate of the mobile phase through the column, which is necessary for efficient separation of compounds. The pressure also helps to ensure that the analytes are pushed through the column at a constant rate, resulting in consistent and reliable chromatographic results. Adequate pressure is needed to overcome frictional forces within the system and to prevent band broadening.
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Why ordinary liquid pump cannot be used in hplc system?
An ordinary liquid pump cannot be used in HPLC systems because HPLC requires pumps that can generate high pressure to push solvents through the column at a constant and precise flow rate. Ordinary pumps do not provide the level of pressure and flow rate control needed for HPLC separations, which can negatively impact the accuracy and reproducibility of chromatographic results. HPLC pumps are specifically designed to handle the high pressures and flow rates required for efficient separations.
What is difference between high pressure liquid chromatography and high performance lequid chromatography?
High pressure liquid chromatography (HPLC) and high performance liquid chromatography (HPLC) are often used interchangeably. HPLC refers to modern liquid chromatography systems with high resolution and efficiency, while high pressure liquid chromatography specifically highlights the use of higher pressures in the system to improve separation and speed. Both terms generally refer to the same chromatographic technique.
What is post run in HPLC?
Post run in HPLC refers to the time after the completion of a chromatographic analysis where the system continues running to ensure that any remaining compounds are fully flushed out of the column and detector to prevent contamination and achieve a clean baseline for subsequent runs. It is an important step to maintain the integrity and performance of the HPLC system.
What type of species can be separated by HPLC but not by gas liquid chromatography?
mixture of enantiomers can be separated by HPLC
What is the function of pump in HPLC?
The pump in HPLC is responsible for delivering the mobile phase at a constant and precise flow rate through the column. This ensures consistent and reproducible separation of compounds in the sample. The pump helps maintain the pressure necessary for optimal chromatographic performance.
What is dead volume HPLC?
The dead volume in HPLC is 137.45. The dead volume in science is used in retention measurements and also in thermodynamic studies and the abbreviation HPLC stands for High Pressure Liquid Chromatography.
How pH play an important role in hplc sepration?
depending on pH resolution is there
What does HPLC stand for?
HPLC stands for High Performance Liquid Chromatography. It is a technique used to separate and analyze components in a liquid mixture based on their interactions with a stationary phase.
What process that uses pressure to separate substances in a solution?
The process you are referring to is likely a type of chromatography, known as high pressure liquid chromatography (HPLC). In HPLC, a liquid mobile phase is passed through a column of stationary phase under high pressure, separating the components of a mixture based on their interaction with the stationary phase.
What is difference between high pressure liquid chromatography and high performance lequid chromatography?
High pressure liquid chromatography (HPLC) and high performance liquid chromatography (HPLC) are often used interchangeably. HPLC refers to modern liquid chromatography systems with high resolution and efficiency, while high pressure liquid chromatography specifically highlights the use of higher pressures in the system to improve separation and speed. Both terms generally refer to the same chromatographic technique.
How do you distinguised np-hplc and rp-hplc?
NP-HPLC (normal phase HPLC) separates compounds based on their polarity, where the stationary phase is polar and the mobile phase is nonpolar. RP-HPLC (reverse phase HPLC) separates compounds based on their hydrophobicity, where the stationary phase is nonpolar and the mobile phase is polar. RP-HPLC is more commonly used due to its versatility and ability to handle a wider range of compounds.
What is difference bet GLC and HPLC?
GLC (Gas Liquid Chromatography) uses gas as the mobile phase and liquid as the stationary phase, while HPLC (High Performance Liquid Chromatography) uses liquid as the mobile phase and a solid or semi-solid stationary phase. GLC is mainly used for volatile compounds, while HPLC is more versatile and can analyze a broader range of compounds.
Why reproducibility is important in hplc?
Reproducibility in HPLC ensures that results can be consistently obtained when the experiment is repeated, leading to reliable data. It allows for verification of results by other researchers and ensures the accuracy and reliability of the method. Reproducibility is crucial for validating the robustness of the HPLC method and for ensuring that results are accurate and can be trusted.
How would forensic scientist detect codeine?
HPLC (high pressure liquid chromatography), hair samples and bodily fluids can be tested this way.
Retention time calculation for hplc?
why RT was shifting & how to RT calculation in HPLC
What is the function of pump in HPLC?
The pump in HPLC is responsible for delivering the mobile phase at a constant and precise flow rate through the column. This ensures consistent and reproducible separation of compounds in the sample. The pump helps maintain the pressure necessary for optimal chromatographic performance.
What type of species can be separated by HPLC but not by gas liquid chromatography?
mixture of enantiomers can be separated by HPLC
