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discussion electrophoretic separation DNA ?
c) probe. A probe is a short stretch of DNA labeled with a radioisotope or fluorescent marker that is used to identify the presence of specific DNA sequences in a sample.
A reporter enzyme monitors transformation of host cells by foreign DNA in addition to a selectable marker through insertional inactivation. It differentiates recombinants from non recombinants.
I hope this will help you: http://www.sfusd.edu/programs/sf_base/protocols/lab_104_lambda.pdf Fun Fact: DNA stands for Deoxyribonucleic acid (But, you probably already knew that).
Blue script plasmid is a type of cloning vector used in molecular biology for DNA cloning. It generally contains elements such as a bacterial origin of replication, a selection marker, and sites for restriction enzyme digestion for inserting foreign DNA. BlueScript plasmids are often used for routine cloning and sequencing purposes.
Dna
DNA passes through a gel at different speeds depending on its size. The purpose of the ladder marker of a DNA is to make the passing of DNA possible.
No
yes
The ladder DNA marker is the reference to indicate the position of a particular resolved band according to the molecular weight. Once the gel is solved, the Rf of the bands can be estimated in reference to the ladder DNA marker.
discussion electrophoretic separation DNA ?
A single peak for a specific STR marker indicates that the individual has two identical alleles at that particular locus. This information can be used in DNA profiling for identification and genetic testing purposes.
c) probe. A probe is a short stretch of DNA labeled with a radioisotope or fluorescent marker that is used to identify the presence of specific DNA sequences in a sample.
A reporter enzyme monitors transformation of host cells by foreign DNA in addition to a selectable marker through insertional inactivation. It differentiates recombinants from non recombinants.
I hope this will help you: http://www.sfusd.edu/programs/sf_base/protocols/lab_104_lambda.pdf Fun Fact: DNA stands for Deoxyribonucleic acid (But, you probably already knew that).
A region of DNA that varies from person to person is called a polymorphic region or a polymorphism. These variations are responsible for the uniqueness and diversity observed among individuals.
DNA bands are usually visualized using techniques such as agarose gel electrophoresis or polyacrylamide gel electrophoresis. After electrophoresis, DNA bands can be viewed under UV light by staining the gel with a fluorescent dye, such as ethidium bromide. The DNA bands will appear as distinct bands of varying sizes depending on the migration pattern of the DNA fragments.