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RafaAdding pH buffers to microbiological media helps maintain a stable pH level, which is critical for the growth and survival of microorganisms. Fluctuations in pH can inhibit the growth of certain organisms or cause changes in their metabolism. Buffers help ensure a consistent environment for microbial growth and reproducibility of experimental results.
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Why we add buffer to standardization of ED-TA solution?
Buffers are added to systems in order to resist any minor changes in pH. EDTA is an acid, (ethylene diamine tetracetic acid), and so a buffer is used in order to maintain a certain pH even after the EDTA is added.
How can one remedy the effect of pH CHANGE due to sterilization?
One way to remedy the effect of pH change due to sterilization is to buffer the solution. Buffers resist changes in pH and can help maintain the desired pH level. Another approach could be to add a small amount of acid or base to adjust the pH back to the desired level. Regular monitoring of pH during and after sterilization can also help in detecting and addressing any changes promptly.
What is chemical composition of tributyrin agar media?
Tributyrin agar media is composed of tributyrin as the main carbon source, agar as the solidifying agent, and water. Tributyrin is a triglyceride consisting of three butyrate molecules attached to a glycerol backbone. This media is often used for the selective growth of bacteria that can hydrolyze or metabolize tributyrin.
What happens in a buffer when the h ions increases?
Normally, when HCl is added to a solution, pH decreases by a large factor. However, because a buffer solution is a weak acid, the effect on the pH on the solution will be considerably less. To understand why, see http://michele.usc.edu/java/acidbase/acidbase.html
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