Light microscopes require light to be reflected from the base of the microscope, up through the organism, and into the eye. If an object you are trying to view is too thick, the light will not pass through the specimen as easily. Also, thicker objects cause more light to refract so that less of it passes through the pinholes, thus making it difficult to focus on.
Here's a helpful website on microscope optics:
http://web.uvic.ca/ail/techniques/scope_basics.html
Confocal Microscopes can focus on thicker images because they use laser technology, and spinning disk confocal microscopes can actually view live cells. However, high magnification on these types of microscopes often requires either a water or oil immersion lens.
When using high magnification, the depth of field decreases, making it challenging to keep all parts of a large organism in focus simultaneously. This is known as the depth of field limitation and requires adjusting the focus manually or using techniques like image stacking to capture sharp details across the entire organism.
At a high magnification, the image of the organism is 'zoomed in', and it is possible to see the image in great detail. However, this means that you're only looking at a very small part of the organism -- in order to see more, you need to 'zoom out'. It's a trade - off between seeing a small area in great detail, or seeing a large area in less detail.
The low powered one because it just is :)
If he/she was looking at something like a plant cell he would use the smaller lens because they would be much larger then something like a cheek cell which would need the medium or large magnification
Keytone Species
false
A dissection microscope is called a stereoscopic microscope. It has low magnification that is useful for viewing large, thick objects.
To bring an object into focus on a microscope, you should start by placing the specimen on the stage and selecting the lowest magnification lens. Next, use the coarse focus knob to move the microscope stage upward until the object is visible, then use the fine focus knob to sharpen the image.
The large knob on the side of a microscope is used for coarse focusing, which moves the objective lens closer or farther from the specimen in larger increments. The small knob is for fine focusing, which allows for precise adjustments to bring the specimen into clear focus.
The power of magnification on a microscope is determined by the combination of the objective lens and the eyepiece lens. The objective lens plays a significant role in magnification by determining the initial magnification of the specimen, while the eyepiece lens further magnifies the image. Different objective lenses have different magnification powers (e.g., 4x, 10x, 40x), and the total magnification is calculated by multiplying the magnification of the objective lens by that of the eyepiece.
Example: 2* magnifacaition makes the focused area twice as large as the actual size.
A course adjustment knob is a control mechanism used in scientific instruments like microscopes or telescopes to make large-scale adjustments to the position or focus of the lens or mirror. It allows for quick and rough changes to the viewing angle or magnification.
A dissection microscope is called a stereoscopic microscope. It has low magnification that is useful for viewing large, thick objects.
it is the course focus
A scanning electron microscope has a very large depth of field which makes the images produced appear three dimensional. Magnifications from 25X to 250,000X (250 times the magnification limit of a light microscope) are possible. Although my Science Text book says it can reach a magnification of 300 000X, but most specimens are easier to view at magnification less than 10 000X.
Advantages: Provides a wider field of view, making it easier to locate objects. Allows for better depth perception and easier focusing. Helps maintain a larger depth of field, making it easier to observe three-dimensional objects. Disadvantages: Lower resolution and image quality compared to higher magnifications. Limited ability to observe fine details and structures. May not be suitable for observing small or intricate objects.
The low powered one because it just is :)
A dissection microscope is called a stereoscopic microscope. It has low magnification that is useful for viewing large, thick objects.
If the surface area of the organism is small, then there is no problem with getting all the oxygen needed. If the surface area of the organism is large, therefore a special respiratory surface is needed. An example is lungs, gills. The ratio of surface area to volume in a small organism is greater than the ratio in a large organism.