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DNA polymerase is an enzyme that finctions best when itis placed in the right ionic environment. All buffers and all other constituents required for the PCR reaction are added first. The buffers ensure the maintainace of the correct ionic environment for the polymerase to function optimally. Therefore, the DNA polymerase is added last AFTER addition of template and primer
DNA polymerase is added last in PCR to prevent it from degrading prematurely due to the high temperatures used in the denaturation and annealing steps. This helps ensure that the polymerase remains active and is able to efficiently synthesize the DNA during each cycle of amplification.
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What chemical is an enzyme that add nucleotides to a new strand of DNA during replication?
DNA Polymerase is the enzyme which adds new nucleotides during replication.
What enzyme binds to DNA during transcription unzipping the strands of RNA?
RNA polymerase is the enzyme that binds to DNA during transcription and helps unzip the DNA strands to allow for the synthesis of RNA.
What enzyme binds to DNA and is responsible for making a transcript RNA of DNA?
RNA polymerase is the enzyme responsible for binding to DNA and synthesizing a complementary RNA strand during transcription.
What is enzyme that adds nucleotides to exposed nitrogen bases?
DNA polymerase is the enzyme that adds complementary nucleotides to exposed nitrogen bases during DNA replication.
What enzymes produced a new DNA strand during DNA replication?
DNA Polymerase
What molecule is active during the last step of replication?
DNA polymerase
What chemical is an enzyme that add nucleotides to a new strand of DNA during replication?
DNA Polymerase is the enzyme which adds new nucleotides during replication.
Two new strands of DNA molecules grow as bases are added by the enzyme?
DNA polymerase during DNA replication. Each new strand is complementary to the original template strand and forms a double helix structure.
Which molecule is active during the last step during DNA replication?
DNA ligase. Apex
Enzyme used to position nucleotides during DNA replication?
DNA polymerase is the enzyme responsible for positioning nucleotides during DNA replication. DNA polymerase can add nucleotides to the growing DNA strand in the 5' to 3' direction and proofread for errors in base pairing.
What enzyme is used to join DNA nucleotides?
In DNA replication, DNA polymerase III is the enzyme which joins the DNA nucleotides together via phospodiester bonds.DNA Ligase is the enzyme that seals gaps in DNA during DNA Replication.DNA Ligase is the enzyme that seals gaps in DNA during DNA Replication.
What enzyme transcribes DNA?
RNA polymerase is the enzyme responsible for transcribing DNA into RNA during the process of transcription.
What is the name of the enzyme that match the DNA bases?
DNA polymerase matches the bases on the parent strand.
What enzyme binds to DNA during transcription unzipping the strands of RNA?
RNA polymerase is the enzyme that binds to DNA during transcription and helps unzip the DNA strands to allow for the synthesis of RNA.
What attaches free nucleotides to the growing DNA strand?
DNA polymerase attaches free nucleotides to the growing DNA strand during DNA replication. DNA polymerase catalyzes the formation of phosphodiester bonds between the nucleotides, incorporating them into the new strand complementing the template DNA.
What enzyme binds to DNA and is responsible for making a transcript RNA of DNA?
RNA polymerase is the enzyme responsible for binding to DNA and synthesizing a complementary RNA strand during transcription.
What is enzyme that adds nucleotides to exposed nitrogen bases?
DNA polymerase is the enzyme that adds complementary nucleotides to exposed nitrogen bases during DNA replication.
