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This can happen because of the pH variation in your dna sample or the chloroform:isoamyl alcohol was not properly removed during the washing step.

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12y ago
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6mo ago

Gel loading dye contains different components such as tracking dyes (bromophenol blue, xylene cyanol), glycerol, and buffers which can give different coloration to DNA samples due to their chemical properties and interactions. The color differences help visualize the DNA movement in the gel during electrophoresis and also indicate the loading efficiency.

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Q: Why gel loading dye give different colouration with DNA sample?
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