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The copper is used to support the polymer layer on which the particles/object of interest is deposited. You need a thin layer of polymer/carbon because in TEM you measure transmitted electrons, so the electrons need to be able penetrate the material on which the sample is deposited. A few micrometers of material can already absorb all the electrons. The copper is just to support this polymer layer and to make a sample which is easy to handle.

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11y ago
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5mo ago

Polymer film helps support the sample, reducing charging effects and improving contrast. Carbon-coated copper grids provide a stable and conductive substrate for mounting and imaging samples, enhancing resolution and reducing contamination in the transmission electron microscope (TEM).

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Q: Why do you use Polymer and carbon coated Copper grids in TEM?
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