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More often than not, a mixture is not in a substance, but rather the substance is in the mixture , often called a solution. Solubility can be seen visibly as thus: homogenous, when the substance and solution are settled together in one state and it looks like a pure substance (for example, dissolving salt in water), and heterogeneous, where the solution and substance are not necessarily in the same state but there are very apparent layers or particles (for example, oil on water).
If your question was, "how to separate a substance in a mixture using the principles of solubility", you would need to determine if the final solution is homogenous or heterogeneous. If it is heterogeneous, like oil on water, you can easily separate it by pouring the oil out. If it is homogenous, like salt in water, you would need to evaporate the water (and perhaps condense it in a separate beaker if you are trying to collect the water), so that only salt is left.
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Why do colours separate in chromatography?
Colors separate in chromatography because different compounds have different affinities for the stationary phase and the mobile phase. As the mobile phase travels through the stationary phase, compounds are carried at different rates based on their interactions with the two phases. This results in the separation of the compounds based on their unique properties.
What is the thin layer chromatography technique used to separate complex mixtures of?
Thin layer chromatography is a technique used to separate complex mixtures of compounds based on their differential partitioning between a stationary phase (thin layer of adsorbent material on a plate) and a mobile phase (solvent moving up the plate). As the mobile phase moves through the stationary phase, compounds in the mixture will separate based on their affinity for the stationary phase and the mobile phase.
What is the name of the technique commonly used to separate different isoenzymes from one another?
The technique commonly used to separate different isoenzymes from one another is called gel electrophoresis. This method takes advantage of each isoenzyme's unique electrophoretic mobility in a gel matrix to separate them based on their size and charge differences.
Which chromatography to separate non volatile compounds?
Column chromatography is commonly used to separate non-volatile compounds based on their interactions with the stationary phase within the column. The compounds are separated as they travel at different rates through the column due to varying affinities to the stationary phase.
Can you separate 2 compounds with similar retention time in a gas chromatography?
Yes, compounds with similar retention times in gas chromatography can be separated by using different stationary phases or adjusting the temperature gradient of the column. Additionally, using a tandem technique like gas chromatography-mass spectrometry (GC-MS) can help in identifying and separating the compounds based on their mass spectra.
