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500 ml

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11y ago
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5mo ago

The working volume in a 500ml shake flask is typically around 20-25% less than the total flask volume, so it would be around 375-400ml. This allows for proper aeration and mixing of the culture without overflowing during shaking.

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Q: What is the working volume in a 500ml shake flask?
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Because you asked how to make a 9 g/L solution of NaCl rather than a 9.0 g/L, or a 9.00 g/L solution, to make a 9 g/L solution of NaCl you first weigh out 9 g of dry NaCl. The weight, actually mass, of the NaCl needs to be 9 g plus or minus 1 g. Next, add the 9 g of NaCl to less than one liter of water, perhaps 25 or 50 mL less. Stir or shake the flask very well for several minutes after all of the NaCl has dissolved, then bring the total volume up to nearly 1 L. Stir or shake the flask very well again, then add water if necessary to ensure that the total volume is one liter.


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