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The site of DNA replication in eukaryotes is the nucleus. Replication occurs in the nucleus because this is where the DNA is stored. The process involves unwinding the DNA double helix and synthesizing new strands of DNA using the existing strands as templates.
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How does topoisomerase affect the DNA strand during DNA replication?
The topoisomerase enzyme uncoils the double helical structure of DNA during its replication to form the replication fork. In eukaryotes both posive and negative supercoils get unbind by topoisomerase I & II respectively.Topoisomerase isomerase unwinds DNA to form replication fork
What sets of materials are required by both eukaryotes and prokaryotes for replication?
Both eukaryotes and prokaryotes require nucleotides (A, T/U, C, G) for DNA replication, DNA polymerase enzymes for synthesizing new DNA strands, and primers to initiate the replication process. Other materials like ATP for energy and various cofactors and proteins are also necessary for efficient DNA replication.
When prokaryotic cells copy their DNA replication begins at?
The origin of replication site, where the DNA unwinds and replication machinery binds to start duplicating the DNA.
The site where the old DNA strands separate and new DNA strands will be synthesized is called the?
The site where the old DNA strands separate and new DNA strands are synthesized is called the replication fork. This is where the enzyme DNA polymerase adds nucleotides to the growing DNA strand.
What is meant by ori region in a plasmid?
The ori region, or origin of replication, in a plasmid is a specific sequence of DNA where replication begins. It is necessary for the plasmid to replicate independently within a host cell. The ori region contains the necessary signals for the initiation of DNA replication.
How does topoisomerase affect the DNA strand during DNA replication?
The topoisomerase enzyme uncoils the double helical structure of DNA during its replication to form the replication fork. In eukaryotes both posive and negative supercoils get unbind by topoisomerase I & II respectively.Topoisomerase isomerase unwinds DNA to form replication fork
What sets of materials are required by both eukaryotes and prokaryotes for replication?
Both eukaryotes and prokaryotes require nucleotides (A, T/U, C, G) for DNA replication, DNA polymerase enzymes for synthesizing new DNA strands, and primers to initiate the replication process. Other materials like ATP for energy and various cofactors and proteins are also necessary for efficient DNA replication.
How does DNA replication differ in prokaryotes and eukaryotes?
1. In eukaryotic cells replication forks make several start sites along the DNA strand which forms replication "bubbles" which get larger the more DNA is copied, and stop when DNA replication is complete. In prokaryotic cell's DNA is formed in a loop, two replication forks start along one part of the loop (origin replication) and the replication forks copy DNA in opposite directions until they meet at the other side of the loop, making an exact copy of DNA.
Why does replication in prokaryotes differs from replication in eukaryotes?
Replication in prokaryotes is simpler and faster due to their single circular chromosome and lack of membrane-bound organelles. Eukaryotes have multiple linear chromosomes and a more complex replication process involving factors like histones, telomeres, and multiple origins of replication. Additionally, eukaryotic cells exhibit more regulatory control over their replication process compared to prokaryotes.
What the nucles manufacture?
neucleus is the site of DNA replication hence it forms DNA
When prokaryotic cells copy their DNA replication begins at?
The origin of replication site, where the DNA unwinds and replication machinery binds to start duplicating the DNA.
Do replication forks speed up replication?
Yes, replication forks do speed up the replication process by allowing DNA synthesis to occur simultaneously in both directions around the circular DNA molecule in prokaryotes or at the two replication forks in eukaryotes. This helps to expedite the replication process and minimize the time needed for DNA replication.
What are enzymes that have a proofreading role in DNA replication?
DNA polymerases, such as DNA polymerase III in prokaryotes and DNA polymerase delta in eukaryotes, have proofreading activities during DNA replication. These enzymes possess exonuclease activity, allowing them to detect and correct errors in newly synthesized DNA strands by removing misincorporated nucleotides. This proofreading function helps maintain the fidelity of DNA replication.
The site where the old DNA strands separate and new DNA strands will be synthesized is called the?
The site where the old DNA strands separate and new DNA strands are synthesized is called the replication fork. This is where the enzyme DNA polymerase adds nucleotides to the growing DNA strand.
What is meant by ori region in a plasmid?
The ori region, or origin of replication, in a plasmid is a specific sequence of DNA where replication begins. It is necessary for the plasmid to replicate independently within a host cell. The ori region contains the necessary signals for the initiation of DNA replication.
What structure in the nucleus holds the DNA?
The DNA molecule forms a double helix. The linear DNA chromosomes of eukaryotes form a highly supercoiled double helix.
What are the areas on DNA where replication occurs called?
DNA replication begins in areas of DNA molecules are called origins of replication.
