0
PEG (polyethylene glycol) is a crowding agent, while isolating plasmid with the traditional common method if use PEG in the last step this increases the concentration of DNA i.e. it removes different ions and impurities from the DNA and make DNA more pure and concentrated. Generally we use PEG solution made up in NaCl or commercially available PEG-8000 NaCl.
Wiki User
∙ 12y ago
Polyethylene glycol (PEG) is often used in transformation to increase the permeability of cell membranes by destabilizing the lipid bilayer. This allows foreign DNA to enter the cells more easily. PEG treatment is commonly used in protoplast fusion and some methods of DNA uptake in plant cells.
Add your answer:
Earn +20 pts
What is the role of Tris-HCl in phage preparation by PEG precipitation?
To Prevent Possible protease contamination
How many ML is a peg measure?
A peg, which when unqulified, refers to a "small peg" is 30 ml.
What is the process in which bacteria take up pieces take up pieces of DNA from their environment?
transformation
Role of peg in plasmid DNA isolation?
PEG (polyethylene glycol) is commonly used in plasmid DNA isolation to precipitate the DNA. When mixed with DNA in a high-salt buffer, PEG causes the DNA to aggregate and precipitate out of solution. This allows for separation of the plasmid DNA from other cellular components, making it easier to purify the DNA.
What is the reaction between PEG and lignocellulose?
PEG can form hydrogen bonds with lignocellulose components such as cellulose and hemicellulose, leading to enhanced compatibility and dispersibility of lignocellulose in water. This interaction can improve the mechanical properties and stability of composites made with PEG and lignocellulose.
What is the role of operations manager in transformation process?
1. role of the operations manager in the transformation process.
What is the role of law in the transformation of an individual and the society?
In Arizona, SB 1307 prohibits the transformation of individuals and the society.
How are expressive skills used in 'the theatre of transformation'?
Theatre transformation is when someone is playing a role but then transforms into something else.
What is the role of Tris-HCl in phage preparation by PEG precipitation?
To Prevent Possible protease contamination
What role do evaporation have in water cycle?
This is the transformation of liquid water in gaseous water.
What is a violin peg called?
a peg. or a tuning peg
Donald Fagen and peg who is she?
Peg is his mom Peg Canales.
What is the role of family in the transformation agenda of president Goodluck Jonathan?
The role of family in the transformation agenda of President Goodluck Jonathan is to help end corruption. The family is the backbone of any country and if the families are transformed then the whole nation would be transformed.
Is peg leg a noun?
Leg is a noun but peg describes it. Peg is an adjective.
The increasing role of in the life course adds to the fluid nature of lifespan development.?
stagewise transformation
What is the role of DMSO in Transformation storage Solution?
it serves as an anti-freezing and cryo-protectant for the cells.
What are the actions of serine dehydratase?
The serine dehydratase is an enzyme; enzymes act as catalysts in biochemical reactions. Role of serine dehydratase: - transformation of serine in pyruvate - transformation of threonine in propionyl CoA
