0
Still curious? Ask our experts.
Chat with our AI personalities
Blake
Vivi
JudyAdd your answer:
Earn +20 pts
How do you separate bromocresol green and methyl red?
One way to separate bromocresol green and methyl red is by using paper chromatography. The two compounds can be spotted onto a piece of chromatography paper and allowed to separate in a solvent. The different solubilities of the compounds will cause them to migrate at different rates, allowing for their separation.
Why does bromocresol green indicator give a different titer value than phenolphthaline indicator?
Bromocresol green changes color over a broader pH range (3.8-5.4) than phenolphthalein (8.2-10.0), leading to a different endpoint in titration. This difference in color change can result in slightly different titer values when using the two indicators.
Why is bromocresol green a good indicator?
Bromocresol green changes color over a pH range of 3.8 to 5.4, making it suitable for acidic to neutral pH ranges. It has a distinct color change from yellow to blue, which is easily visible to the naked eye. Additionally, it is stable and has a long shelf life.
How does colour changing bubble bath work?
The bubble bath has a dye added to it called Bromocresol Green which is a pH indicator, it will change colour as you change the pH of the bathwater - how acid or alkali the solution is. (Interestingly the one colour it never goes is green!)So as you dilute the bubble bath, which starts off slightly acid, the pH then gets higher and the water becomes more alkaline, making the Bromocresol Green change from orange to blue. If the water becomes acid again it will change from blue back to orange.
What was the purpose of examining the original pGLO solution with and without UV illumination?
The purpose was to observe the differences in fluorescence levels between the pGLO solution under normal conditions and when exposed to UV light. This allowed for visual confirmation of the presence and activation of the GFP gene in the pGLO solution when exposed to UV light.
