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How do you separate bromocresol green and methyl red?
One way to separate bromocresol green and methyl red is by using paper chromatography. The two compounds can be spotted onto a piece of chromatography paper and allowed to separate in a solvent. The different solubilities of the compounds will cause them to migrate at different rates, allowing for their separation.
Why does bromocresol green indicator give a different titer value than phenolphthaline indicator?
Bromocresol green changes color over a broader pH range (3.8-5.4) than phenolphthalein (8.2-10.0), leading to a different endpoint in titration. This difference in color change can result in slightly different titer values when using the two indicators.
Why is bromocresol green a good indicator?
Bromocresol green changes color over a pH range of 3.8 to 5.4, making it suitable for acidic to neutral pH ranges. It has a distinct color change from yellow to blue, which is easily visible to the naked eye. Additionally, it is stable and has a long shelf life.
How does colour changing bubble bath work?
The bubble bath has a dye added to it called Bromocresol Green which is a pH indicator, it will change colour as you change the pH of the bathwater - how acid or alkali the solution is. (Interestingly the one colour it never goes is green!)So as you dilute the bubble bath, which starts off slightly acid, the pH then gets higher and the water becomes more alkaline, making the Bromocresol Green change from orange to blue. If the water becomes acid again it will change from blue back to orange.
What was the purpose of examining the original pGLO solution with and without UV illumination?
The purpose was to observe the differences in fluorescence levels between the pGLO solution under normal conditions and when exposed to UV light. This allowed for visual confirmation of the presence and activation of the GFP gene in the pGLO solution when exposed to UV light.
What color would a bromocresol green solution be at pH 7?
Bromocresol green is a pH indicator that changes color based on the pH of the solution. At pH 7, bromocresol green would be blue in color.
What is the color of the antacid solution or bromocresol purple when an antacid is no longer effective?
The antacid solution or bromocresol purple will turn yellow when the antacid is no longer effective. This change in color indicates that the solution has become more acidic, suggesting that the antacid is no longer effective in neutralizing stomach acid.
3 natural indicators which can be used to detect basic solution?
Examples: turmeric, methyl red, bromocresol green.
What is the pka of bromocresol green?
The pKa of bromocresol green is around 4.7.
What is the principle of bromocresol purple in albumin test?
Bromocresol purple is a pH indicator dye that changes color based on the pH of a solution. In an albumin test, bromocresol purple is used to detect the presence of albumin in urine or serum by changing color from yellow to purple in the presence of albumin. This color change occurs because albumin binds to the dye molecule, causing a shift in pH that results in the color change.
What is the purpose of using the blank solution?
The blank solution is used to be compared with a solution containing a component to be determined; the blank solution is supposed to be uncontaminated by this substance. The blank solution is important for the calibration of instruments.
How do you separate bromocresol green and methyl red?
One way to separate bromocresol green and methyl red is by using paper chromatography. The two compounds can be spotted onto a piece of chromatography paper and allowed to separate in a solvent. The different solubilities of the compounds will cause them to migrate at different rates, allowing for their separation.
How do you prepare bromocresol green and methly red indicator?
To prepare bromocresol green indicator, dissolve 0.1 g of bromocresol green in 100 mL of ethanol. For methyl red indicator, dissolve 0.1 g of methyl red in 100 mL of ethanol. Store both indicators in a tightly sealed container away from light.
Why does bromocresol green indicator give a different titer value than phenolphthaline indicator?
Bromocresol green changes color over a broader pH range (3.8-5.4) than phenolphthalein (8.2-10.0), leading to a different endpoint in titration. This difference in color change can result in slightly different titer values when using the two indicators.
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