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1.Take 8 to 12 test tubes and label them..
2. Add 5 ml saline in each tube to provide isotonic medium..
3.Add 5 cc blood sample in each tibe
4.Add 1 ml solution containing antibodies
5.Mix and shake gently
5.Took half of first test tube and add to 2nd tube then took half of 2nd tube and add to 3rd tube and son...It is serial dilution..Leave the last tube as such .dnt add antibodies in it..It is Control
6.Keep all test tubes in water bath at temp 37 deg C for 30 min
7.refrigerate them overnight at 4 deg C
8.Check agglutination in the tubes caused by Ag - Ab complex
9. Check the last tube having agglutination..Suppose its dilution is 1:32,then the titer of Ab will b 32..
BY:::dr sakhawat ali
In a hemagglutination test, red blood cells are mixed with a test substance (such as a virus or antibody) and agglutination (clumping) is observed. The test helps determine the presence or quantity of certain substances in a sample, based on the agglutination pattern. The procedure involves preparing the red blood cells, adding the test substance, and interpreting the results based on the degree of agglutination.
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Hemagglutination-inhibition test description?
The hemagglutination-inhibition (HI) test is a laboratory technique used to detect antibodies against a specific virus, such as influenza. It involves mixing the virus with red blood cells and antibodies, which inhibit the virus from agglutinating the red blood cells. By measuring the inhibition of hemagglutination, the presence and titer of antibodies against the virus can be determined.
The procedure indicates that the amount of time th test tube was kept in th boiling water bath should be minimized why was this necessary?
The procedure indicates that the amount of time the test tube was kept boiling in the water bath should be minimized. The water bath should never be allowed to boil dry.
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Hemagglutination-inhibition test description?
The hemagglutination-inhibition (HI) test is a laboratory technique used to detect antibodies against a specific virus, such as influenza. It involves mixing the virus with red blood cells and antibodies, which inhibit the virus from agglutinating the red blood cells. By measuring the inhibition of hemagglutination, the presence and titer of antibodies against the virus can be determined.
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