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Why does catalase elute in a wider range of fractions than glucose oxidase does?
Catalase may elute in a wider range of fractions than glucose oxidase due to differences in their molecular weights, hydrophobicity, and interactions with the gel filtration or chromatography resin. Catalase is a larger and more complex protein compared to glucose oxidase, which can lead to a broader elution profile. Additionally, catalase may have different binding affinities or interactions with the resin, resulting in varied elution behavior.
Why is GOD reagent specific to detection of glucose?
GOD (glucose oxidase) is specific to detecting glucose because it specifically catalyzes the oxidation of glucose to gluconic acid while reducing molecular oxygen to hydrogen peroxide. This reaction is unique to glucose and does not occur with other sugars, making GOD a specific enzyme for glucose detection.
Which is the enzyme which catalyses the reaction of glucose to gluconic acid?
Glucose oxidase that converts the carbonyl (aldehyde) carbon of glucose to a carboxylic acid.
What is the molecular weight of Dextrin?
The average molecular weight of dextrin can vary depending on the degree of polymerization and branching. Typically, dextrin has a molecular weight ranging from 500 to 5,000 g/mol.
What is the molecular formula of the principal molecular or ionic species present in glucose?
It is a molecular species with the formula C6H12O6
Why does catalase elute in a wider range of fractions than glucose oxidase does?
Catalase may elute in a wider range of fractions than glucose oxidase due to differences in their molecular weights, hydrophobicity, and interactions with the gel filtration or chromatography resin. Catalase is a larger and more complex protein compared to glucose oxidase, which can lead to a broader elution profile. Additionally, catalase may have different binding affinities or interactions with the resin, resulting in varied elution behavior.
Glucose molecular weight in Dalton unit?
The molecular weight of glucose is approximately 180.16 daltons.
How does glucose react with glucose oxidase?
Glucose reacts with glucose oxidase by binding to the enzyme's active site. Glucose oxidase then catalyzes the oxidation of glucose to produce gluconic acid and hydrogen peroxide. This reaction can be used to detect or quantify glucose levels in various samples.
What kind of macromolecule is insulin?
Glucose is grouped as a Carbohydrate.
Why divide glucose mgs by 180 for millimoles?
Because the formula for calculating moles is dividing grams by the molecular weight. The molecular weight for glucose is 180. grams to moles is also the same as milligrams to millimoles.
What molecule is larger glucose or albumin?
Albumin is a larger molecule compared to glucose. Albumin is a protein with a molecular weight around 66-69 kDa, while glucose is a small molecule with a molecular weight of 180 g/mol.
Equivalent weight of glucose?
The equivalent weight of glucose is 180 g/mol. This is calculated by dividing the molecular weight of glucose (180 g/mol) by the number of equivalents of reactive atoms in the compound, which in this case is 1.
The molecular weight of free glucose is 180.2 while that of glucose in glycogen is 160 explain this anomaly with structural diagrams?
The difference in molecular weight is due to glycogen being a polymer made up of many glucose molecules linked together, with the addition of branching points. This structure results in a lower average molecular weight compared to free glucose, which is a single glucose molecule. The branching points in glycogen reduce the overall mass of the molecule per glucose unit, leading to the lower molecular weight of glycogen.
Why is GOD reagent specific to detection of glucose?
GOD (glucose oxidase) is specific to detecting glucose because it specifically catalyzes the oxidation of glucose to gluconic acid while reducing molecular oxygen to hydrogen peroxide. This reaction is unique to glucose and does not occur with other sugars, making GOD a specific enzyme for glucose detection.
If the atomic weight of carbon is twelve and the atomic weight of oxygen is sixteen the molecular weight of glucose is?
The chemical formula of glucose is C6H12O6. To find the molecular weight, you need to add up the atomic weights of each atom in the formula: 6 carbons (12 each), 12 hydrogens (1 each), and 6 oxygens (16 each). The total molecular weight of glucose is 6(12) + 12(1) + 6(16) = 180.
Which is the enzyme which catalyses the reaction of glucose to gluconic acid?
Glucose oxidase that converts the carbonyl (aldehyde) carbon of glucose to a carboxylic acid.
What do the two molecules ( fructose and glucose ) have in common?
Fructose and Glucose are isomers of each other. That means that one part of the molecule is in a different location on the other molecule. Fructose and glucose have the same molecular formula and molecular weight.
