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Q: What is the essence of using flame in every inoculation experiment?
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The bottom of a test tube was covered with soot after an experiment which required heating. What kind of flame was used during the experiment?

A yellow, smoky flame was likely used during the experiment, which produced soot as a byproduct of incomplete combustion. This type of flame is often seen with a fuel-rich mixture or inefficient burning of the fuel.


Provide two examples of how the Bunsen burner is used during inoculation of a tube culture?

The Bunsen burner is used to sterilize the inoculating loop or needle before and after transferring the culture to prevent contamination. The Bunsen burner flame can also be used to heat the neck of the tube culture to create an updraft that prevents airborne contaminants from entering the tube during inoculation.


How did Joseph Priestly relight the candle in his experiment?

Joseph Priestley relit the candle by placing a mint leaf near the flame, which resulted in the flame rekindling due to the release of oxygen from the mint leaf during photosynthesis. This experiment demonstrated the concept of oxygen being necessary for combustion.


When the Bunsen burner is on but no experiment is being carried out why is it advisable to set it to the luminous flame?

Setting the Bunsen burner to the luminous flame when not in use helps prevent soot buildup on the burner. The luminous flame produces less heat and is less likely to cause accidental burns or ignition of nearby materials in case of a mishap. Additionally, it reduces the risk of gas leakage since the flame is more stable and less likely to flicker.


Which elements give off a color when heated and what color?

Different elements produce different colors when heated. Here are a few examples: Lithium produces a red flame Sodium produces a yellow flame Copper produces a blue-green flame Potassium produces a lilac flame Barium produces a pale green flame

Related questions

Why must it e flamed after making an inoculation?

Flaming the inoculation loop or needle is essential to sterilize it and prevent the introduction of contaminants into the culture medium. This helps ensure that only the desired microorganism is being transferred during the inoculation process, minimizing the risk of unwanted contamination in the culture.


The bottom of a test tube was covered with soot after an experiment which required heating. What kind of flame was used during the experiment?

A yellow, smoky flame was likely used during the experiment, which produced soot as a byproduct of incomplete combustion. This type of flame is often seen with a fuel-rich mixture or inefficient burning of the fuel.


What is Bunsen Burner used for?

A Bunsen burner produces heat and creates a heat source when doing an experiment. It uses methane and there is two flames a safety flame-you can put your hand through it without burning your hand and a roaring flame-this flame is blue and it is what you use when you preform an experiment.


Why a luminous flame is not used as a source of heat in a laboratory experiment?

A luminous flame produces soot, which can contaminate the experiment or react with chemicals being used. In addition, the temperature of a luminous flame is not as high or consistent as a non-luminous flame, making it less suitable for precise heating applications in a laboratory setting.


What is the size and length of inoculation loop?

Inoculation loops can be purchased in different sizes, either in metal for flame sterilisation or in plastic for use with pathogens which are disposed of after a single use. Typically they range from 0.25 - 0.5cm internal diameter.


Why is it important to do the experiment with the blue flame first?

Doing the experiment with the blue flame first is important because it helps establish a baseline understanding of how a clean flame behaves and what to expect as a reference point. This can help in identifying anomalies or deviations when conducting experiments with other flames later on.


When would you use a blue flame in a lab situation?

when you are doing an experiment that involves "the effect of heat on a ..."


What is a Controlled variable in a flame test?

A controlled variable in a flame test is a factor that is kept constant throughout the experiment to ensure that only the variable being tested (the element or compound producing the flame color) is affecting the outcome. Examples of controlled variables in a flame test include the type of burner used, the temperature of the flame, and the type of chemical used to clean the loop.


How do you perform cold flame experiment with phosphorus?

To perform the cold flame experiment with phosphorus, you need white phosphorus, a glass container, a beaker of water, and safety equipment. Submerge the glass container in water to eliminate any air. Cut a small piece of white phosphorus and ignite it inside the container. The phosphorus will burn, producing a cold, greenish-white flame due to the presence of phosphorus pentoxide. Remember to wear appropriate safety gear and perform the experiment in a well-ventilated area.


Why is it advice to work near the flame during aseptic technique?

It is advice to work near a flame during aseptic technique because it has to be performed under sterile conditions. Working near a flame allows for flame sterilization which would promote sterilization to the experiment you are working on.


The olympic flame is relite every how many years?

About every 2 years.


What flame should the Bunsen burner be left on?

The Bunsen burner should be left on a low flame to prevent accidents. It should be adjusted based on the specific experiment being conducted to ensure the desired heat level is maintained.