0
Coconut water can serve as a source of nutrients and growth factors in tissue culture medium, supporting the growth and development of plant cells and tissues. Its natural composition of sugars, vitamins, and minerals can help enhance cell proliferation and organogenesis in vitro. Additionally, the cytokinins present in coconut water can stimulate shoot formation and overall growth in plant tissue cultures.
Coconut water and Cytokinin
in the history of orchid micropropagatiion, western scientist did not use it, since there is no coconut tree in Europe. But in Java Island, scientist who works in the Bogor (Buitenzorg) Botanical Garden in Indonesia; become acquinted with CW (coconut water; colorless liquid of coconut endosperm) not CM (coconut milk= santan in Indonesian) ie; Johannes van Overbeek.
He and Mr M E Conklin, suggested it's use to Albert Blakeslee for the culture of immature embryos of Datura stramonium. The embrios grew well in the presence of CW and and an efficient complex additive was introduced into plant tissue culture. Nowadays, CW is used widely in tissue culture and micro propagation of many plants
Add your answer:
Earn +20 pts
What media does Chlamydia pneumoniae grow on?
Chlamydia pneumoniae grows on specialized cells called epithelial cells, typically found in the respiratory tract. These bacteria are obligate intracellular pathogens, meaning they require living host cells to replicate and survive.
What is definition of ex-plant in plant tissue culture?
In plant tissue culture, ex-plant refers to the part of the plant that is used to initiate tissue culture. This can be any part of the plant, such as a leaf, stem, or root, that is sterilized and placed in a nutrient medium to grow into a new plant. The ex-plant serves as the starting material for tissue culture propagation.
Why the callus is first transferred to rooting medium in plant tissue culture?
Transferring the callus to a rooting medium in plant tissue culture helps induce the differentiation of callus cells into roots. The rooting medium is usually supplemented with hormones that promote root formation, leading to the development of a full plant with a well-established root system that can be successfully transplanted into soil for growth and further development.
What type of culture medium is agar beef heart and human blood?
Agar beef heart and human blood is a selective culture medium that is specific for detecting certain bacteria, such as fastidious organisms that require nutrients found in blood and heart tissue to grow. This medium is commonly used for isolating and identifying pathogens like Haemophilus influenzae.
Why sterile conditions are needed in tissue culture?
The conditions that allow you to culture tissue are also ideal for the growth of bacteria, which given a chance will devour your tissue culture.
What are the main function of the agar in the plant tissue culture medium?
Agar in plant tissue culture medium serves as a solidifying agent, providing a gel-like matrix for tissue support and growth. It also helps in the absorption and retention of water and nutrients by the plant tissues. Additionally, agar helps prevent contamination and provides a stable environment for tissue culture growth.
Why use 5 percent CO2 in tissue culture?
The addition of 5% CO2 in tissue culture creates a more physiologically relevant environment for cell culture by maintaining the pH of the culture medium and promoting cell growth. This level of CO2 represents a balance between providing enough CO2 for cellular respiration and avoiding excessive acidification of the medium.
What media does Chlamydia pneumoniae grow on?
Chlamydia pneumoniae grows on specialized cells called epithelial cells, typically found in the respiratory tract. These bacteria are obligate intracellular pathogens, meaning they require living host cells to replicate and survive.
Why do people plant coconut palms?
Coconuts are used for a number of things: The oil is used for drinking, hair tonic and even as a medium in tissue culture. The coconut "meat" is used for eating as well as dried and using in baking and cooking. The coconut husk (fibre around the seed) is used for to produce mates, brooms and brushes, the by-product of this is used as a growing medium for plants and seedlings (coconut "peat"). The trunk of the palm (when it dies) is used for a number of things including containers, fibre and decoration. The plants themselves are used both for fruit production as well as their use in ornimental landscaping
Which tissue in husk of coconut?
Sclerenchyma
Is micro propagation is method of tissue culture?
Yes, micropropagation is a method of tissue culture where small pieces of plant tissue are cultured in a nutrient medium to produce multiple identical plants. This technique is commonly used for rapid propagation of plants.
What is definition of ex-plant in plant tissue culture?
In plant tissue culture, ex-plant refers to the part of the plant that is used to initiate tissue culture. This can be any part of the plant, such as a leaf, stem, or root, that is sterilized and placed in a nutrient medium to grow into a new plant. The ex-plant serves as the starting material for tissue culture propagation.
Are tissue culture and plant tissue culture same?
In plant tissue culture, cells of plants are cultured. In tissue culture, cells (of plants, animals, bacteria, etc.) are cultured. Plant tissue culture is just like a subheading under tissue culture
Why medium is considered to be very important in tissue culture technique?
In tissue culture, the medium also called the sterile jelly is very essential because it provides all the necessary nutrients , hormones , enzyme which is needed for the growth and development of the tissue and helps it to produce many cells which form a shapeless mass called a 'callus' .This then could be transferred to another jelly which helps it to develop roots and another to develop shoot which then could be transplanted to pots or soil which helps it to mature and grow to form new plant . hence medium is considered very important in tissue culture technique.
Why the callus is first transferred to rooting medium in plant tissue culture?
Transferring the callus to a rooting medium in plant tissue culture helps induce the differentiation of callus cells into roots. The rooting medium is usually supplemented with hormones that promote root formation, leading to the development of a full plant with a well-established root system that can be successfully transplanted into soil for growth and further development.
What type of culture medium is agar beef heart and human blood?
Agar beef heart and human blood is a selective culture medium that is specific for detecting certain bacteria, such as fastidious organisms that require nutrients found in blood and heart tissue to grow. This medium is commonly used for isolating and identifying pathogens like Haemophilus influenzae.
Do coconut milk help grow new plants from old plants because it contains?
Coconut milk alone is not typically used to grow new plants from old ones. While it does contain some nutrients that could potentially support plant growth, it is not a common or recommended method for plant propagation. Typically, plants are propagated using methods such as cuttings, division, or seeds.
