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Bacterial smears that are undergoing some type of differential staining method are stained with a primary dye. Primary dye typically refers to a Crystal-voilet stain that is used in the gram staining procedure to color all cells purple. After a mordant (iodine-makes the dye complex less soluble), decolorizer, and a counterstain are added to the primary dye, a person can view the slide under a microscope in order to distinguish gram-positive bacteria from gram-negative bacteria. The cell wall of gram-negative bacteria will not retain the primary dye and be washed away with the decolorizer. Gram-neg. bacteria will then take on the counterstain providing contrast between the two types of bacteria when viewed under a microscope.

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13y ago
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5mo ago

In histology, tissue samples are stained with a primary dye like hematoxylin or eosin to highlight different structures and components. Hematoxylin stains nuclei blue, while eosin stains cytoplasm and extracellular matrix pink, aiding in the visualization and analysis of tissue under a microscope.

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Q: What is stained with primary dye?
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