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Heavy DNA is DNA that is labeled with the heavy isotope of Nitrogen, 15N
Brian Udashkin, Woodridge NY
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∙ 13y ago
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Meselson and Stahl used what type of isotopes to determine mechanism by which DNA replicated?
Meselson and Stahl used heavy isotopes of nitrogen (N^15 and N^14) to track the replication of DNA in E. coli bacteria. By switching from N^15 to N^14, they were able to demonstrate the semi-conservative mechanism of DNA replication.
In a gel electrophoresis's why are the lines closer together when the DNA strands are heavier?
Heavier DNA strands move slower through the gel due to their larger size and mass, causing them to travel shorter distances during the same amount of time compared to lighter DNA strands. This results in the heavier DNA bands being closer together on the gel, as they have not traveled as far as the lighter bands in the same time frame.
What is CsCl profile of a DNA and why CsCl is used for ultracentrifugation?
Since CsCl is a kind of heavy salt, it forms density gradients when centrifugation. When sedimentation equilibrium, DNA moves to the position where meets its density. The density of DNA is related to its GC%, by analytical ultracentrifugation, we can read the GC levels of the DNA from its position in the solution. Please refer to the article for detail: Using analytical ultracentrifugation to study compositional variation in vertebrate genomes (Euro Biophys J. 2003 32: pp418-426)
What is the function of DNA helicare in DNA replication?
DNA Helicase unwinds and unzips the DNA. It separates the two strands of DNA so DNA replication can occur.
Why is recombinant DNA a new form of DNA?
Recombinant DNA is a new form of DNA because it is created via introduction of the relevant DNA into the existing organismal DNA.
Meselson and Stahl used what type of isotopes to determine mechanism by which DNA replicated?
Meselson and Stahl used heavy isotopes of nitrogen (N^15 and N^14) to track the replication of DNA in E. coli bacteria. By switching from N^15 to N^14, they were able to demonstrate the semi-conservative mechanism of DNA replication.
In a gel electrophoresis's why are the lines closer together when the DNA strands are heavier?
Heavier DNA strands move slower through the gel due to their larger size and mass, causing them to travel shorter distances during the same amount of time compared to lighter DNA strands. This results in the heavier DNA bands being closer together on the gel, as they have not traveled as far as the lighter bands in the same time frame.
What is CsCl profile of a DNA and why CsCl is used for ultracentrifugation?
Since CsCl is a kind of heavy salt, it forms density gradients when centrifugation. When sedimentation equilibrium, DNA moves to the position where meets its density. The density of DNA is related to its GC%, by analytical ultracentrifugation, we can read the GC levels of the DNA from its position in the solution. Please refer to the article for detail: Using analytical ultracentrifugation to study compositional variation in vertebrate genomes (Euro Biophys J. 2003 32: pp418-426)
How is DNA tested and why?
by DNA fingerprinting method , DNA-DNA hybirdization or DNA sequencing. to know the sequence of DNA
What checks DNA for error?
DNA fingerprinting
How would the DNA of the human compare to the DNA of the duck?
DNA is DNA it can not be changed.
What are 5 harmful metals and why?
Two are Lead and Mercury these can cause nerve and brain damage and are classified as heavy metal poisons. Chromium, and especially some of its compounds can cause cancer. Copper, if ingested, can form oxidizing agents that damage lipids, proteins, and DNA. Uranium is both a heavy metal poison and is radioactive.
What is a DNA molecule containing regions from different sources?
A DNA molecule containing regions from different sources is called recombinant DNA. This is often created in laboratories by combining DNA from different organisms or through genetic engineering techniques. Recombinant DNA technology has many applications in biotechnology and genetic research.
What enzyme binds fragments of DNA on the lagging strand?
DNA ligase is the enzyme that binds together the Okazaki fragments on the lagging strand during DNA replication. It forms phosphodiester bonds between adjacent nucleotides to create a continuous strand of DNA.
What is the function of DNA helicare in DNA replication?
DNA Helicase unwinds and unzips the DNA. It separates the two strands of DNA so DNA replication can occur.
What is a synonym for DNA replication?
IT can be called * DNA duplication * DNA reproduction * DNA imitation
The cells hereditary material?
DNA
