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Broth dilution is a method used in microbiology to determine the minimum inhibitory concentration (MIC) of an antimicrobial agent. It involves serially diluting the antimicrobial agent in liquid growth medium (broth) containing the bacteria being tested. The lowest concentration of the antimicrobial agent that inhibits visible bacterial growth is considered the MIC.
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How do you do a serial dilution of a specific concentration of culture?
bacterial cell numbers needs reducing ,which is done by repeatedly diluting the amount of you have in your sample. A small amount of bacterial sample is mixed with a diluent solution(such as sterile broth), and then dilution are made. by adding small amount of diluted bacteria samples then spread onto the agar plate by L-shaped glass rod.
What is the key idea to remember when considering the dilution of a solution?
The key idea to remember when considering the dilution of a solution is that the amount of solute remains constant before and after dilution. Therefore, the concentration of the solute decreases as more solvent is added. The equation C1V1 = C2V2 is commonly used to calculate the new concentration or volume after dilution.
Is dilution is the addition of solute to decrease the concentration of solvent?
No. dilution is the addition of solvent to decrease the concentration of a solute.
Why moles solute before dilution equals moles solute after dilution?
This assumes you are using water (or whatever the original solvent is) to do the dilution so you are not adding more solute, only more solvent,
What is broth microdilution method CLSI M27 A2?
It is a standard method defined in the M27-A2 documentation from the National Committee for Clinical Laboratory Standards. The method is for antifungal susceptibility testing and determines the minimum inhibitory concentration, which is the concentration of the antifungal in a dilution series distributed in culture broth wells, where there is no growth of the tested organism.
