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An EMB (Eosin Methylene Blue) plate is a selective and differential agar medium used for the isolation and differentiation of Gram-negative bacteria, particularly for detecting Enterobacteriaceae. It contains lactose, eosin Y, methylene blue, and peptone to inhibit the growth of Gram-positive bacteria and selectively isolate Gram-negative bacteria. The medium distinguishes lactose fermenters (which appear pink to dark purple) from non-fermenters (which appear colorless or pale).

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Q: What is an EMB plate?
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What does Proteus mirabilis look like on the EMB plate?

Yes it does


How to Tell the difference between E. coli and enterobacter aerogenes?

when you plate E.coli onto a EMB plate the colonies turn shinie metalic green, EMB is selective for gut bacteria and it diferentiates E.coli.


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Do EMB plate dyes inhibit growth of Gram-positive or negative bac?

EMB plate dyes inhibit the growth of Gram-positive bacteria and promote the growth of Gram-negative bacteria. The dyes in EMB plates help differentiate between the two types of bacteria based on their ability to ferment lactose. Gram-negative bacteria that ferment lactose will produce dark colonies with a metallic green sheen.


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14k EMB means made of solid 14k gold andembossed or embedded with another matterial (enamel, gemstones...)


Does bacillus grow on EMB agar?

Bacillus bacteria typically do not grow well on Eosin Methylene Blue (EMB) agar because they are gram-positive bacteria. EMB agar is designed to inhibit the growth of gram-positive bacteria and isolate gram-negative bacteria, such as Escherichia coli.


Will staph grow on EMB agar?

Staphylococcus bacteria typically do not grow well on Eosin Methylene Blue (EMB) agar. EMB agar is selective for gram-negative bacteria, particularly Enterobacteriaceae, and contains dyes that inhibit the growth of gram-positive bacteria like Staphylococcus.


Will pseudomona aeruginosa grow on Emb?

Pseudomonas aeruginosa can grow on Emb agar, as it is a selective and differential growth medium commonly used to isolate and differentiate members of the Enterobacteriaceae family, including P. aeruginosa. Its growth on Emb agar will exhibit the characteristic green metallic sheen due to its ability to produce pyocyanin pigment.


What is the difference between EMB agar and MacConkey agar?

EMB agar is selective for Gram-negative bacteria and can differentiate between lactose fermenters and non-fermenters based on colony color. MacConkey agar is selective for Gram-negative bacteria and contains lactose and pH indicators to differentiate between lactose fermenters (pink colonies) and non-fermenters (colorless colonies).


What type of bacteria grow on EMB?

Eosin methylene blue (EMB) agar is selective for gram-negative bacteria, especially those that can ferment lactose. Common bacteria that grow on EMB agar include Escherichia coli and Enterobacter aerogenes. These bacteria are differentiated by their ability to ferment lactose, producing colonies that are metallic green or dark purple on EMB agar.