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Not 100% sure, but I believe it's a primer with a linker attached to it. A linker is a short DNA that has a restriction site built-in. Imagine this: you want to amplify a gene by PCR, but it has no suitable restriction site for you to clone into a vector. So by PCR with a fusion primer (with built-in restriction site), you can simply digest the ends and ligate it into your vector
Wiki User
∙ 12y ago
A fusion primer is a short DNA sequence that contains regions complementary to two adjacent DNA segments that are intended to be joined together. It enables the specific amplification of the target DNA region during PCR by priming the synthesis of the DNA strand that bridges the two segments. This technique is commonly used in molecular Biology to create gene fusions or chimeric constructs.
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What is the best primer?
The best primer for you will depend on your skin type and concerns. A good general option is a hydrating primer for dry skin, a mattifying primer for oily skin, or a blurring primer for minimizing pores and imperfections. It's always best to try samples or get recommendations from a makeup artist to find the best primer for your specific needs.
In polymerase chain reaction how many kinds of primer are used?
In polymerase chain reaction (PCR), two types of primers are used: a forward primer and a reverse primer. These short DNA sequences are specific to the target DNA region to be amplified and serve as starting points for DNA synthesis by the DNA polymerase enzyme.
When was aluminium wood primer first released?
Aluminum wood primer was first developed in the 1960s as a paint primer specifically formulated for use on wood surfaces.
How do you stop rust after sandblasting to bare steel?
To stop rust after sandblasting to bare steel, you can apply a primer specifically designed to prevent rust, such as a zinc-rich primer or an epoxy primer. Make sure the primer is applied evenly and thoroughly to create a protective barrier on the exposed steel surface. Topcoat the primer with a high-quality paint to provide an additional layer of protection against moisture and corrosion.
Which nuclear fusion cycle is the next one to begin after helium fusion ends in a massive star?
The next nuclear fusion cycle after helium fusion in a massive star is carbon fusion. This process involves fusing helium nuclei to form carbon. Carbon fusion typically occurs in the core of a massive star after helium fusion is completed.
What is the best paint primer for plastic?
There is a brand of paint sold in most hardware outlets for painting plastic or vinyl . This paint does not need primer first. It is called Krylon Fusion.
What primer can you use on copper?
Etch primer
What is the best primer?
The best primer for you will depend on your skin type and concerns. A good general option is a hydrating primer for dry skin, a mattifying primer for oily skin, or a blurring primer for minimizing pores and imperfections. It's always best to try samples or get recommendations from a makeup artist to find the best primer for your specific needs.
What is a writer's primer?
A primer is a book of elementary principles. A writer's primer, therefore, would be the principles of writing as a craft.
How do you mixomni 2k primer?
to thin 2k primer or primer fillers such as dupont 1040r a raitio of 5:1 is used ie: 500mls primer to 100mls activator, if the mixture needs to be thinned, first mix the primer and activator to above ratio then add thinners at 20mls of thinners per 100mls primer mix. eg: 500mls primer +100mls activator= 600mls, 6x20mls=120mils of thinners for 600mils primer mix.
Is oil primer better than acrylic primer in bathrooms?
no its not
When was Primer - video - created?
Primer - video - was created in 1989.
What primer is needed at the origin of nucleotide addition?
A primer made of RNA is required at the origin of nucleotide addition for DNA replication. This primer provides a free 3' OH group for DNA polymerase to start adding nucleotides and serves as a starting point for DNA synthesis.
When was Primer Impacto created?
Primer Impacto was created in 1994.
When was Sylvester Primer born?
Sylvester Primer was born in 1842.
When did Sylvester Primer die?
Sylvester Primer died in 1912.
When was Primer - album - created?
Primer - album - was created in 1995.
