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What ingrediant could be eliminated if the medium was strictly used for indole production?
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∙ 6y ago
Trytophan
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∙ 10y ago
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What is the component in the SIM deep tubes that makes the medium suitable to detect the production of indole by bacteria?
The component in SIM deep tubes that makes the medium suitable to detect the production of indole by bacteria is tryptophan. Bacteria that possess the enzyme tryptophanase can break down tryptophan to produce indole, which can be detected by the addition of Kovac's reagent that reacts with indole to form a red color.
What is the component in the SIM deep tubes that makes this medium suitable to detect the production of indole?
The component in the SIM deep tubes that makes them suitable for detecting indole production is tryptophan. Tryptophan is an amino acid that can be broken down by certain microorganisms to produce indole, which can then react with Kovac's reagent to produce a pink-red color. This color change is used as a positive indicator of indole production.
Principle of ehrlich's test?
Ehrlich's test is a presumptive test for the presence of indole compounds, such as tryptophan, in a sample. It relies on the reaction between indole compounds and p-dimethylaminobenzaldehyde to produce a blue-colored complex. This test is commonly used in microbiology to detect the indole production by bacteria such as Escherichia coli.
What are the indole test principles?
The indole test is based on the ability of certain bacteria to produce the enzyme tryptophanase, which converts tryptophan into indole. This indole is then detected by adding a reagent such as Kovac's reagent, which reacts with indole to produce a red color. A positive test indicates the presence of tryptophanase enzyme in the bacteria.
Why determine motility and h2s before adding kovac's reagent?
Determining motility involves observing the movement of bacteria, which can be hindered by adding Kovac's reagent as this reagent can affect bacterial motility. Also, hydrogen sulfide production (H2S) is an important characteristic that can help differentiate bacterial species before adding Kovac's reagent, which is used to detect indole production. Waiting to add Kovac's reagent until after these tests ensures more accurate results.
What is Staphylococcus aureus sim test results?
Staphylococcus aureus is negative for motility, and positive for the indole production. The SIM test tests for sulfur reduction, indole production and motility. Positive for indole production means tryptophan is broken down into indole and pyruvate and will give a red color. Motility, you will see the bacteria move outside of the stab. Positive for sulfur reduction will give a black color in the medium.
What factors dictate the choice of test in a combination medium?
Factors such as the specific bacteria being tested, the purpose of the test (identification or enumeration), the characteristics of the medium (selectivity, differential, enrichment), and the expected response or outcome of the test play a role in determining the appropriate test to use in a combination medium. Different tests can provide different types of information about the bacteria present in a sample, so choosing the right test is important for accurate and reliable results.
What is the component in the SIM deep tubes that makes the medium suitable to detect the production of indole by bacteria?
The component in SIM deep tubes that makes the medium suitable to detect the production of indole by bacteria is tryptophan. Bacteria that possess the enzyme tryptophanase can break down tryptophan to produce indole, which can be detected by the addition of Kovac's reagent that reacts with indole to form a red color.
What factors dictate the choice of tests included in a combination medium?
The hydrogen sulfide production, motility, and indole production.
Does proteus vulgaris produce h2s?
Yes, it does. It is Indole Production positive, H2S positive, and Motility positive. This can all be seen using SIM agar. Yes, it does. It is Indole Production positive, H2S positive, and Motility positive. This can all be seen using SIM agar.
What is SIM tube media used for?
SIM (Sulfide-Indole-Motility) tube media is used to differentiate bacteria based on their ability to produce hydrogen sulfide, indole, and exhibit motility. It contains ferrous sulfate to detect hydrogen sulfide production, tryptophan to test for indole production, and a semi-solid agar to assess motility. This media is commonly used in microbiology labs to identify and characterize microorganisms.
Is staphylococcus epidermidis indole positive?
No, Staphylococcus epidermidis is usually indole negative, meaning it does not produce indole as a byproduct of tryptophan metabolism. This can help differentiate it from other bacteria that are indole positive.
What is the component in the SIM deep tubes that makes this medium suitable to detect the production of indole?
The component in the SIM deep tubes that makes them suitable for detecting indole production is tryptophan. Tryptophan is an amino acid that can be broken down by certain microorganisms to produce indole, which can then react with Kovac's reagent to produce a pink-red color. This color change is used as a positive indicator of indole production.
Principle of ehrlich's test?
Ehrlich's test is a presumptive test for the presence of indole compounds, such as tryptophan, in a sample. It relies on the reaction between indole compounds and p-dimethylaminobenzaldehyde to produce a blue-colored complex. This test is commonly used in microbiology to detect the indole production by bacteria such as Escherichia coli.
What is an indole alkaloid?
class of alkaloids containing a structural moiety of indole.
What does the S.I.M. test for?
Sulfur Indole Motility test
What test will differentiate between Proteus vulgaris and Citrobacter freundii?
Indole test,Proteus vulgaris is be indole positive and Citrobacter freundii will be indole negative
