Wiki User
∙ 7y agoThe molar extinction coefficient (also sometimes called molar absorbtivity coefficient) is a measure of how strongly a solution of a substance absorbs light (the value depends on the particular wavelength of light used). By passing light through a solution and determining how much of the light is absorbed, you can use the path length and molar extinction coefficient to determine the concentration of the solution.
Look up "Beer-Lambert law" if you want details.
Wiki User
∙ 7y agoThe molar extinction coefficient of BSA (bovine serum albumin) is approximately 43,824 M^(-1)cm^(-1) at a wavelength of 280 nm. This value is commonly used to quantify the concentration of BSA in a solution based on its absorbance at 280 nm.
We would need to know the path length and the molar extinction coefficient to answer that question. If you know these, it's an extremely simple matter of Beer's Law and algebra.
the empirical formula and the molar mass
To calculate 1 millimolar (mM) concentration of a substance, you need to know the molar mass of the substance. Then, divide 1 millimole (mmol) by the molar mass to obtain the volume of the substance needed to make a 1 mM solution.
To calculate the number of moles of oxygen in the reaction vessel, you need to know the amount of oxygen in grams and its molar mass. Then divide the mass of oxygen by its molar mass to obtain the number of moles. The formula to calculate the number of moles is: moles = mass / molar mass.
15.4 * 103 at 260 nm pH 7.5
Molar extinction coefficient of phenol ret at 610nM is 22 mM-1 cm-1
Molar extinction coefficient is depend on intensity of the colour of solution.If the solution has high intensity of colour, molar extinction coefficient is high.So when considering CoCl2 and KMnO4, CoCl2 has low colour intensity. KMnO4 solution has much intense purple colour.Therefore its Molar extinction coefficient is high. By-Tharindu Chathuranga Ariyathilaka/Sri Lanka
The molar extinction coefficient of BSA (bovine serum albumin) is approximately 43,824 M^(-1)cm^(-1) at a wavelength of 280 nm. This value is commonly used to quantify the concentration of BSA in a solution based on its absorbance at 280 nm.
In the beginning, no you need not cull the special coefficient
The extinction coefficient can refer to a few different measures how strongly a distinct medium absorbs light at a particular wavelength. The two most commonly referred to are molar absorptivity (which measures absorption per molar concentration) and the mass attenuation coefficient (which measures absorption per mass density).
The molar extinction coefficient of ADP at a specific wavelength varies depending on the solvent and experimental conditions. It is typically around 8100 M-1cm-1 at 259 nm for ADP in water. Make sure to consult a reliable source or perform your own measurements for accurate values.
You might get an aproximate answer with the formula here. http://www.proteinscience.org/cgi/reprint/4/11/2411.pdf
I am not some one of this background and so please correct me if I am wrong. I think molar extinction coefficient will be very less and according to molecular structure of any molecule (bond vibration etc..,) only some wavelengths will have reasonable absorption and for glucose those wavelenghts are 1550-1850 nm; 6450-5400 cm(-1) (first overtone) 2000-2500 nm; 4000-5000 cm(-1) (combination). These are the spectral windows in which glucose has significant absorption.
The molar mass is the measurement of mass per one mole of a substance. If the mass of a matter is known, divide it from the molar mass to obtain the number of moles.
To find the molar proportions of each oxide in a chemical compound, you first determine the molar masses of each element present in the compound. Then, calculate the molar ratio of each element by dividing their molar masses by the smallest molar mass. Finally, simplify the ratios to whole numbers if necessary to obtain the molar proportions.
When you substitute the unit of grams for amu (atomic mass unit), you obtain the molar mass of the compound in grams per mole. This molar mass represents the mass of one mole of the compound, which is a fundamental concept in chemistry for measuring amounts of substances.