The cytotoxic t cell directly attacks and lyses cellular pathogens. They are activated by antigens that stimulate an immune response.
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One mechanism that directly attacks and lyses cellular pathogens is the complement system. This system consists of a group of proteins that can form pores on the cell membranes of pathogens, leading to cell lysis. Additionally, certain immune cells, such as natural killer cells or cytotoxic T cells, can release toxic substances that directly kill pathogens.
When a cell lyses, its membrane breaks open, causing the cell to burst and release its contents into the surrounding environment. This can occur due to physical damage, osmotic stress, or the actions of certain enzymes or toxins.
Sodium deoxycholate is a detergent commonly used in cell culture media to disrupt lipid membranes and solubilize membrane proteins. It helps in cell lysis, protein extraction, and is often used in combination with other detergents to maintain the solubility of membrane proteins in solution.
The host cell lyses, which means the host cell burst and is killed.
Enzymes called lysozymes can lyse bacterial cell walls by breaking down the peptide bonds in the peptidoglycan layer. Other substances, such as detergents or antibiotics, can also disrupt and lyse cell walls by affecting membrane integrity or cell wall synthesis.
DNAzol is a reagent used in DNA extraction to lyse cells by disrupting the cell membrane and nucleus. It helps release DNA from the cells and proteins, allowing for subsequent separation and purification of the DNA. DNAzol also helps protect the DNA from degradation during the extraction process.