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Restriction enzymes (REN's) cut or cleve DNA. This cutting process is sequence-specific. Which means, the enzyme does not randomly fragment the DNA (or template strand as it is generally called in many experimental protocols). Instead, the enzyme scans the length of the template until it reaches a specific sequence of 4 - 8 nucleotides. Once this sequence (the restriction site) is reached, the enzyme produces a cut in the template and generates either blunt ends (no overhangs) or sticky ends (having both 3' and 5' overhangs)
The primary application of restriction enzymes is is genetic engineering where we want to insert cDNA sequences into plasmids or other vectors.
Wiki User
∙ 15y ago
Restriction enzymes are used in DNA cloning to cut DNA at specific recognition sites, in restriction fragment length polymorphism (RFLP) analysis to study genetic variations, and in gene editing techniques like CRISPR to modify specific sequences in the genome.
Wiki User
∙ 13y agoUse them to identify people or finger prints ( like in a crime scene)
for cloning DNA
and for sequencing DNA
:)
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