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The term protoplast refers to the spherical shape assumed by Gram-positive bacteria. Spheroplast refers to the spherical shape assumed by Gram-negative bacteria. The difference is essentially the presence of a single membrane, in the case of the protoplast, and the two membranes (inner and outer) of the Gram-negative spheroplasts. It is also possible to generate a gram-negative protoplast by the removal of the outer membrane. Thus, in essence, protoplast refers to a bacterial sphere that is bounded by a single membrane and spheroplast refers to a sphere that is bounded by two membranes

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Protoplasts are plant or bacterial cells whose cell wall has been chemically removed, leaving behind the plasma membrane. Spheroplasts are formed when the cell wall of a bacterial cell is partially removed, but not completely as in the case of a protoplast. Both are used in genetic engineering and fusion studies due to their easier ability to take up foreign DNA compared to cells with intact cell walls.

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Q: What are the difference between protoplast and spheroplast?
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Related questions

What is protoplast and spheroplast?

a protoplast is aliving substance of the cell. it has the same consistency with that of the egg and is a colloidal substance with a sol-gel manner.


What is a spheroplast?

It is a type of insect that looks like a cow.


Is the green colour pigment in the leaves called protoplast?

No, it is not protoplast


What is Protoplast Fusion in Becterial cell?

it is a technique by which protoplast cells are fused to get new hybrid.


What is spheroplast cells?

cell that has completely remove cell wall by action of penicillin


What is the difference between protoplast and protoplasm?

Protoplast is spherical completely cell wall deficient form assumed by gram(+) bacteria.It is produced by mechanical or enzymatic action.Presence of only single membrane.It cant be transformed to spheroplast.It can be used for membrane biology,DNA transformation. Whereas spheroplasts are almost completely cell wall deficient form assumed by gram(-) bacteria.It is produced by the action of penicillin.Presence of two membranes-outer & inner.It may possible to generate a gram(-)protoplast by removal of outer membrane.It is used for study of ion channels through patch clamp,transfection.


How would you use the word protoplast in a sentence?

A protoplast has had its cell wall entirely removed, leaving it naked and vulnerable before a lusty audience.


What is found in plant cell?

Cell wall and protoplast


What do you call a cell wall without a chloroplast?

Protoplast ,,


What is the connection of protoplast with cell?

liquid content of the cell


What are the functions of protoplast?

Protoplasts are plant or bacterial cells with the cell wall removed. They are commonly used in genetic engineering, plant breeding, and cell fusion experiments due to their ability to take up foreign DNA, enabling gene transfer and genetic modifications. Protoplast regeneration can also be used in plant transformation and micropropagation techniques.


What structure is found in cells?

Protoplast is found in all cells