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it is not the acid ether concentration technique which produces four layers, but the formalin-ether concentration technique, which, at the end of the procedure after centrifugation will produce an upper layer of ether, a second layer of fat debris, a third layer of formalin and the bottom layer of sediments which contain your eggs and cysts...

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14y ago
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7mo ago

The four layers obtained in the acid ether concentration technique are the aqueous layer, the organic layer, the acid layer, and the ether layer. Each layer contains different components based on their solubility in the respective solvent.

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Q: What are the 4 layers obtained in the acid ether concentration technique?
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