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What are restriction enzymes Explain the significance of these enzymes in recombinant DNA technology.?
Wiki User
∙ 14y ago
A restriction enzyme (or restriction endonuclease) is an enzyme that cuts double-stranded or single stranded DNA at specific recognition nucleotide sequences known asrestriction sites....................refer in this website en.wikipedia.org/wiki/Restriction_enzyme
Wiki User
∙ 14y ago
Restriction enzymes are proteins that cut DNA at specific sequences. They are important in recombinant DNA technology because they allow scientists to isolate specific genes or DNA sequences and insert them into other organisms' DNA. This enables the creation of transgenic organisms with modified or new genetic traits for research, medicine, or agriculture.
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Explain the role of klenow fragment in recombinant DNA technology?
The Klenow fragment, derived from the DNA polymerase I enzyme, is used in recombinant DNA technology to fill in the single-stranded DNA gaps left in a vector after annealing with a DNA insert. It possesses 5' to 3' polymerase activity and 3' to 5' exonuclease activity, allowing it to extend the DNA strands in a template-directed manner. This helps to create recombinant DNA molecules with high efficiency.
Linked genes never separate True or False?
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When you are asked to explain the significance of something, you are being prompted to clarify its importance or relevance. This typically involves highlighting the impact, implications, or relevance of the subject matter in a clear and concise manner.
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The rationale of a study is the reason behind why the research is being conducted. It should explain the significance of the research question, outline gaps in current knowledge, and justify the need for the study to be conducted to advance scientific understanding.
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Explain how DNA technology transfer bacterial genes from cell to cell?
I think you must rethink about your question, but still I am giving the answer as I can understand that you are asking about recombinant DNA technology where bacterial DNA is used as it is a cloning vector (plasmid). In recombinant DNA technology the particular sequence of DNA that we want to replicate or want to produce in huge number, is attached either with plasmid of bacteria or a DNA of bacteriophage and thus produce the recombinant or hybrid DNA which is copied each time when the bacteria or bacteriophage multiply. In this way the hybrid DNA will be transferred from parent cell to daughter cells.
Explain the role of klenow fragment in recombinant DNA technology?
The Klenow fragment, derived from the DNA polymerase I enzyme, is used in recombinant DNA technology to fill in the single-stranded DNA gaps left in a vector after annealing with a DNA insert. It possesses 5' to 3' polymerase activity and 3' to 5' exonuclease activity, allowing it to extend the DNA strands in a template-directed manner. This helps to create recombinant DNA molecules with high efficiency.
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Explain the Molecular mechanism functions of restriction endonucleases?
Restriction Endonucleases recognize certain sites on the DNA or the sequences. For example EcoR1 that recognizes the restriction site GAATTC on any strand of DNA or RNA.
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