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A plasmid is a piece of circular DNA. These plasmids are transformed into bacteria/yeast.
A library is made by taking pieces of DNA (from a genomic DNA digest, or cDNA) and inserting them into plasmids. The plasmids are then transformed into the organism and stored. Libraries are used to screen for new functional genes, usually when looking at new substrates or products that the yeast/bacteria can't make without the plasmid.
Plasmid libraries are collections of plasmids, which are small, circular DNA molecules that are separate from the chromosomal DNA present in bacterial cells. These libraries can contain a variety of cloned DNA fragments or genes that are stored in individual plasmids, making it easier to study, manipulate, and amplify specific DNA sequences. Plasmid libraries are commonly used in molecular Biology research for various applications, such as gene expression studies, functional genomics, and gene editing.
A plamid library is a collection of DNA fragments cloned into a plasmid vector (a typically circular DNA element that can be replicated in a bacterial host and carry an inserted sequence). Escherichia coli bacteria are transformed with the collection of plasmids and single colonies (each containing a different insert) are grown and stored frozen separately. This allows isolation of a plasmid with a specific sequence for further analysis. The library can be used e.g. for screening, i.e. looking for plasmids that contain inserts with a specific property that can be detected by the screen.
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Plasmid libraries are collections of hundreds or thousands of plasmids into which genes of another organism have been inserted True or False?
Due to the fact that the prime [modern day engineered] purpose of plasmids are to transfer Dna, and considering the Rate that we are producing transgenic creatures using plasmids - we have got to go with 'True'.
What is a helper plasmid?
A helper plasmid is a type of plasmid used in molecular biology to aid the replication and maintenance of another plasmid within a host cell. It often contains genes necessary for the replication or transfer of the target plasmid, and can provide other functions such as antibiotic resistance or visualization markers.
What is the term for a plasmid that contains a foreign gene?
Recombiant DNA
What occurs first in the production of a recombinant plasmid?
In the production of a recombinant plasmid, the DNA of interest (insert) and the plasmid vector are both cut with restriction enzymes to create compatible ends. These cut fragments are then ligated together using DNA ligase to produce the recombinant plasmid.
When is a plasmid considered a recombinant plasmid?
A plasmid is considered recombinant when it contains DNA sequences from two different sources that have been artificially combined, often through genetic engineering techniques like restriction enzyme digestion and ligation. This results in a plasmid with modified or additional genetic material compared to its original form.
What are some of the characteristics of cosmid?
A cosmid has the characteristics of a hybrid plasmid. They are used in the building of genomic libraries. They are ~200 base pairs long. More detailed information is available on Wikipedia.
Plasmid libraries are collections of hundreds or thousands of plasmids into which genes of another organism have been inserted True or False?
Due to the fact that the prime [modern day engineered] purpose of plasmids are to transfer Dna, and considering the Rate that we are producing transgenic creatures using plasmids - we have got to go with 'True'.
What are BACs and YACs?
BAC = Bacterial Artificial Chromosome -200-300Kb -good for genomic libraries -uses the bacterial F (sex) plasmid -currently quite popular YAC = Yeast Artificial Chromosome -1Mb -good for genomic libraries also -difficult to manipulate and use -less popular
Which is the plasmid that increases resistance to antibiotics?
R-plasmid
What plasmid that can degrade toluic acid?
TOL plasmid
How can i know if my bacteria contain plasmid or not?
You can determine if your bacteria contain a plasmid by performing a plasmid extraction followed by gel electrophoresis to visualize the presence of plasmid DNA. Other methods include PCR amplification of plasmid-specific sequences or using molecular biology techniques like restriction enzyme digestion to confirm the presence of a plasmid.
What is a self transmissible plasmid?
A plasmid which encodes genes for its own transfer.
A plasmid is a type of?
Plasmid is extrachromosomal DNA capable of self replication.
What is a helper plasmid?
A helper plasmid is a type of plasmid used in molecular biology to aid the replication and maintenance of another plasmid within a host cell. It often contains genes necessary for the replication or transfer of the target plasmid, and can provide other functions such as antibiotic resistance or visualization markers.
How many plasmid slots can you have in BioShock 2?
You can have a maximum of 8 plasmid slots.
What is the term for a plasmid that contains a foreign gene?
Recombiant DNA
What is plasmid curing?
Plasmid curing is the process of obviating the plasmid encoded functions such as antibiotic resistance, virulence, degradation of aromatic compounds, etc. in bacteria. Several plasmid curing agents have been reported in literature, however, no plasmid curing agent can eliminate all plasmids from different hosts.
