A plasmid is a piece of circular DNA. These plasmids are transformed into bacteria/yeast.
A library is made by taking pieces of DNA (from a genomic DNA digest, or cDNA) and inserting them into plasmids. The plasmids are then transformed into the organism and stored. Libraries are used to screen for new functional genes, usually when looking at new substrates or products that the yeast/bacteria can't make without the plasmid.
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∙ 13y agoPlasmid libraries are collections of plasmids, which are small, circular DNA molecules that are separate from the chromosomal DNA present in bacterial cells. These libraries can contain a variety of cloned DNA fragments or genes that are stored in individual plasmids, making it easier to study, manipulate, and amplify specific DNA sequences. Plasmid libraries are commonly used in molecular Biology research for various applications, such as gene expression studies, functional genomics, and gene editing.
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∙ 13y agoA plamid library is a collection of DNA fragments cloned into a plasmid vector (a typically circular DNA element that can be replicated in a bacterial host and carry an inserted sequence). Escherichia coli bacteria are transformed with the collection of plasmids and single colonies (each containing a different insert) are grown and stored frozen separately. This allows isolation of a plasmid with a specific sequence for further analysis. The library can be used e.g. for screening, i.e. looking for plasmids that contain inserts with a specific property that can be detected by the screen.
Due to the fact that the prime [modern day engineered] purpose of plasmids are to transfer Dna, and considering the Rate that we are producing transgenic creatures using plasmids - we have got to go with 'True'.
A helper plasmid is a type of plasmid used in molecular biology to aid the replication and maintenance of another plasmid within a host cell. It often contains genes necessary for the replication or transfer of the target plasmid, and can provide other functions such as antibiotic resistance or visualization markers.
Recombiant DNA
In the production of a recombinant plasmid, the DNA of interest (insert) and the plasmid vector are both cut with restriction enzymes to create compatible ends. These cut fragments are then ligated together using DNA ligase to produce the recombinant plasmid.
A plasmid is considered recombinant when it contains DNA sequences from two different sources that have been artificially combined, often through genetic engineering techniques like restriction enzyme digestion and ligation. This results in a plasmid with modified or additional genetic material compared to its original form.
A cosmid has the characteristics of a hybrid plasmid. They are used in the building of genomic libraries. They are ~200 base pairs long. More detailed information is available on Wikipedia.
Due to the fact that the prime [modern day engineered] purpose of plasmids are to transfer Dna, and considering the Rate that we are producing transgenic creatures using plasmids - we have got to go with 'True'.
BAC = Bacterial Artificial Chromosome -200-300Kb -good for genomic libraries -uses the bacterial F (sex) plasmid -currently quite popular YAC = Yeast Artificial Chromosome -1Mb -good for genomic libraries also -difficult to manipulate and use -less popular
R-plasmid
TOL plasmid
Plasmid is extrachromosomal DNA capable of self replication.
A plasmid which encodes genes for its own transfer.
A helper plasmid is a type of plasmid used in molecular biology to aid the replication and maintenance of another plasmid within a host cell. It often contains genes necessary for the replication or transfer of the target plasmid, and can provide other functions such as antibiotic resistance or visualization markers.
Plasmid curing is the process of obviating the plasmid encoded functions such as antibiotic resistance, virulence, degradation of aromatic compounds, etc. in bacteria. Several plasmid curing agents have been reported in literature, however, no plasmid curing agent can eliminate all plasmids from different hosts.
In the production of a recombinant plasmid, the DNA of interest (insert) and the plasmid vector are both cut with restriction enzymes to create compatible ends. These cut fragments are then ligated together using DNA ligase to produce the recombinant plasmid.
You can have a maximum of 8 plasmid slots.
A plasmid is considered recombinant when it contains DNA sequences from two different sources that have been artificially combined, often through genetic engineering techniques like restriction enzyme digestion and ligation. This results in a plasmid with modified or additional genetic material compared to its original form.