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The limitation of a simple stain are its borders. Whether rigid or in liquid form, stains will only penetrate cloth and textiles to a certain limit. This includes their spread ratio, which will recede once the stain has hits its formation point. Simple stains may also be removed with cleaning solutions and liquids.

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Q: What are merits and limitations of simple staining techniques?
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What are the advantages of differential staining procedures over simple staining techniques?

Using multiple stains can better differentiate between different microorganisms or structures/cellular components of a single organism.


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The result of simple staining of microorganisms is that they can be identified and studied under a microscope.


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Yes, it can


Why is time an important factor in simple staining?

In Gram staining, some bacterial species will become Gram variable meaning they produce a false reaction for the following reasons: decolrized too long, too young/old of culture, stained too long. Also, time is important simply because you don't want to over or under stain. You won't be able to see your specimen as well.


What are the release dates for Simple Techniques in Shaping Glass - 1973?

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