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I have already made ELIAS a lot of times but on the dilution of Ag repeatedlied No matter how much times of OD value are diluted do not have apparent reduction at the same time Under the circumstances?
Wiki User
∙ 15y ago
Is your OD close to max, close to background or very low?
Wiki User
∙ 15y ago
It sounds like you may be experiencing non-specific binding or other interfering factors in your ELISA. You can try optimizing your blocking steps, using high-quality reagents, adjusting incubation times, and possibly trying a different blocking buffer. It may also be helpful to include appropriate controls in your experiment and carefully troubleshoot each step of the assay to identify the issue.
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What is the key idea to remember when considering the dilution of a solution?
The key idea to remember when considering the dilution of a solution is that the amount of solute remains constant before and after dilution. Therefore, the concentration of the solute decreases as more solvent is added. The equation C1V1 = C2V2 is commonly used to calculate the new concentration or volume after dilution.
Is dilution is the addition of solute to decrease the concentration of solvent?
No. dilution is the addition of solvent to decrease the concentration of a solute.
Why moles solute before dilution equals moles solute after dilution?
This assumes you are using water (or whatever the original solvent is) to do the dilution so you are not adding more solute, only more solvent,
What is infinite dilute solution?
Infinite dilution means such a large dilution so that when you add more solvent there is no change in concentration.
What is the recommended dilution for mouthwash?
The recommended dilution for mouthwash is typically 1 part mouthwash to 1 part water. This dilution helps to reduce the intensity of the mouthwash while still maintaining its effectiveness in killing bacteria and freshening breath.
What is the function of medicine lipiget atorvastatin 10mg?
high cholesteol dilution and reduction
What is the effect of dilution on viscosity of oil?
dilution will reduce the viscosity The effect of dilution on viscosity of oil is that it will decrease.
What sentence has the word dilution in it?
yummy dilution water
Definition of dilution and dilution factor?
Dilution is the process of reducing the concentration of a substance by adding more solvent. The dilution factor is the ratio of the final volume of the diluted solution to the initial volume of the concentrated solution. It is used to calculate the new concentration after dilution.
When was Purity Dilution created?
Purity Dilution was created in 1989.
What is the dilution ratio for glyfos weedkiller?
what is dilution rate for glycos
To prepare 300 ml of a 115 dilution from a 13 dilution you need to add ml of the 13 dilution to ml of diluent?
33,4ml
What is the difference between dilute and dilution?
what is the difference between a diffusion and a dilution
What does 0 percent dilution mean?
0 percent dilution means that no additional solvent or liquid has been added to a solution. The solution remains in its original concentration without any dilution.
What is another term for dilution?
Dilution may also be described as 'watering down' or 'mixing', as well as 'diffusing', although none are exact synonyms of dilution.
WHY dilution increase conductivity?
Molar conductivity is what increases dilution. It is the conductivity of an electrolyte solution.
What dilution to make 50percent acetone control?
To make a 50% acetone control, you can mix equal parts of acetone and water. For example, if you start with 10 ml of acetone, you would add 10 ml of water to make a 50% acetone solution.
