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MTT assay is used to measure cell viability by assessing metabolic activity, while crystal violet staining is used to quantify cell proliferation by staining total cellular biomass in a population. Choose MTT assay when you want to measure cell viability specifically, and crystal violet staining when you want to evaluate overall cell proliferation in a population. Each method provides different information about cell health and growth.

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Q: How to choose using an MTT assay vs Crystal Violet Staining?
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Is assay by mass balance equivalent to assay by as is basis?

No, assay by mass balance and assay by as is basis are not equivalent. Assay by mass balance calculates the amount of a component based on material balance equations, while assay by as is basis measures the amount of a component without accounting for any changes or losses that may occur during processing.


What is an assay?

An assay is a scientific test or analysis used to measure the presence, amount, or activity of a substance or compound in a sample. Assays are commonly used in biology, chemistry, and medicine to study a wide range of biological and chemical processes.


How do you assay thrombin potency?

You can assay thrombin potency by measuring its ability to convert fibrinogen to fibrin, typically using a chromogenic or clot-based assay. The results are compared to a standard curve to determine the potency of the thrombin sample.


What is the difference between assay on as is basis and assay on anhydrous basis?

Assay on as is basis refers to the percentage of a substance in its current state, including any impurities or water content present. Assay on anhydrous basis, on the other hand, refers to the percentage of a substance calculated without including any water content present. This difference is important when comparing the purity of substances or calculating accurate concentrations in chemical analysis.


What is percent RSD limit for assay as per USP?

The USP (United States Pharmacopeia) specifies that the relative standard deviation (%RSD) for an assay should generally be less than 2%. However, this limit may vary depending on the specific requirements of the assay and the drug being tested.

Related questions

What is protein assay?

Protein assay is the determination of concentration or total level of protein in a solution.There are various protein assays employed like bradford assay and lowry assay


Is assay by mass balance equivalent to assay by as is basis?

No, assay by mass balance and assay by as is basis are not equivalent. Assay by mass balance calculates the amount of a component based on material balance equations, while assay by as is basis measures the amount of a component without accounting for any changes or losses that may occur during processing.


How do you verify gold bar assay certificate?

You can't verify assay certificates.


When was US Assay Commission created?

United States Assay Commission was created in 1792.


When did US Assay Commission end?

United States Assay Commission ended in 1980.


Can you put the word assay in a sentence?

The old miners in gold rush country took their nuggets to the local Assay Office, to determine -- to assay -- the value of the metal in their nuggets.


What is a bandshift assay?

A bandshift assay is a type of assay using gel electrophoresis, in which the mobility of a DNA or RNA probe alone is compared to its mobility in combination with a particular protein.


What ia the role of normal melting agarose in comet assay?

Normal melting agarose is used in comet assay to create a solid gel matrix in which DNA fragments can migrate based on their size. This agarose helps to separate and visualize DNA fragments, allowing for the detection of DNA damage in individual cells. The agarose gel also serves to protect the DNA during electrophoresis and staining steps.


What is Procedure of assay test of sodium acid citrate?

assay test of sodium acid citrate


What is the budget of United States Assay Commission?

The budget of United States Assay Commission is 2,500 dollars.


Asay un fruit boost assay pillule pour le regime?

assay boost fait mincir?


What does the assay office in Birmingham do?

Assay Offices test precious metals (gold, silver and platinum) and apply hallmarks.