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What would happen if a plasmid was cut at more than one site?
If a plasmid is cut at more than one site by restriction enzymes, it would result in multiple DNA fragments. These fragments can be ligated back together in different combinations, resulting in plasmids with different sizes or configurations. This can lead to the creation of recombinant plasmids with altered properties compared to the original plasmid.
How many times EcoR1 cuts the DNA to generate 4 fragment?
EcoR1 cuts double-stranded DNA at specific recognition sites generating two fragments, so to generate 4 fragments, EcoR1 would need to cut the DNA twice.
How many amino acid fragments will be produced from this polypeptide if it is incubated with a mixture of trypsin and v8 protease?
It depends on the specific cleavage sites for trypsin and V8 protease within the polypeptide sequence, as well as the length and composition of the polypeptide. Generally, these proteases cleave at specific amino acid residues, resulting in smaller peptide fragments. The number of fragments would need to be determined by analyzing the sequence of the polypeptide and the cleavage specificity of the proteases.
Suppose you had 500 pieces of each of the four fragments how would the gel appear?
If you had 500 pieces of each of the four fragments, the gel would show four distinct bands of equal intensity on the gel. The fragments would migrate based on their sizes, with the smallest fragments traveling the farthest and the largest fragments staying closest to the well.
Where large fragments of DNA would be found?
If you mean in fossils or decomposed bodies, it would be best found in the teeth.
If you have a restriction enzyme that cuts a piece of DNA at two recognition sites how many DNA fragments would you see on a gel?
Three.To see why, cut a piece of string in two places! Of course, strictly you would not be able to see only three fragments. You would amplify the DNA before carrying out electrophoresis. That way, you would get perhaps 200 million copies of each fragment, and they would show up. Also, you would only be able to distinguish the fragments if they were different lengths. Electrophoresis separates pieces of DNA by length.
Which enzyme would cut the human DNA shown in Part A on both sides of the vgp gene but not inside the gene?
The enzyme that would cut the human DNA on both sides of the vgp gene but not inside the gene is a restriction enzyme. These enzymes recognize specific DNA sequences and cut the DNA at or near those sequences. By choosing a restriction enzyme that has recognition sites flanking the vgp gene, you can selectively cut the DNA on both sides of the gene without disrupting the gene itself.
What economics problems would result from DDOS attacks launched by terrorists on major e-commerce sites?
The sites would not be able to trade, putting everything at a stand-still.
What would happen if a plasmid was cut at more than one site?
If a plasmid is cut at more than one site by restriction enzymes, it would result in multiple DNA fragments. These fragments can be ligated back together in different combinations, resulting in plasmids with different sizes or configurations. This can lead to the creation of recombinant plasmids with altered properties compared to the original plasmid.
How many times EcoR1 cuts the DNA to generate 4 fragment?
EcoR1 cuts double-stranded DNA at specific recognition sites generating two fragments, so to generate 4 fragments, EcoR1 would need to cut the DNA twice.
Where are all the idol fragments in fossil fighters?
all the idol fragments would be by in the building by the bridge
If you took a linear piece of DNA and cut it with the restriction enzyme EcoRI and it had three restriction sites for EcoRI, how many fragments would you produce What if you had a circular piece of DNA?
If the plasmid has 3 recognition sequences for a given restriction endonuclease, then 4 linear DNA fragments are obtained because, if the DNA is linear then the number of fragments obtained is (N+1) whereas if the DNA is circular then the number of fragments obtained will be N for N recognition sequences for the given restriction endonuclease in a plasmid.
How many amino acid fragments will be produced from this polypeptide if it is incubated with a mixture of trypsin and v8 protease?
It depends on the specific cleavage sites for trypsin and V8 protease within the polypeptide sequence, as well as the length and composition of the polypeptide. Generally, these proteases cleave at specific amino acid residues, resulting in smaller peptide fragments. The number of fragments would need to be determined by analyzing the sequence of the polypeptide and the cleavage specificity of the proteases.
Suppose you had 500 pieces of each of the four fragments how would the gel appear?
If you had 500 pieces of each of the four fragments, the gel would show four distinct bands of equal intensity on the gel. The fragments would migrate based on their sizes, with the smallest fragments traveling the farthest and the largest fragments staying closest to the well.
Why don't rock fragments follow the same path in the rock cycle?
Rock fragments can take different paths in the rock cycle due to various factors such as erosion, deposition, heat, and pressure. These processes can result in the transformation of rock fragments into different types of rocks, ultimately leading to their divergent pathways in the cycle. Additionally, the influence of external factors like water, wind, and temperature variations can also contribute to the unique journey of rock fragments in the rock cycle.
What would have to happen to granite for it to become a sedimentary rock?
It would have to be weathered & the fragments cemented together.
Where large fragments of DNA would be found?
If you mean in fossils or decomposed bodies, it would be best found in the teeth.
