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The KIA medium has an iron salt-ferric ammonium citrate- and sodium thio sulfate. The ferric ions bind with the hydrogen sulfide to then form ferrous sulfide, which gives off a black percipitate that can be seen on the media.

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In Kia medium, hydrogen sulfide (H2S) production can be demonstrated by the formation of a black precipitate. This occurs when H2S reacts with iron salts in the medium to form iron sulfide, which is black in color. The presence of this black precipitate is a positive indicator of H2S production in the KIA medium.

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Q: How is hydrogen sulfide demonstrated in KIA medium?
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What is a Kliger's iron agar and SIM have in common?

Kliger's iron agar (KIA) and SIM (sulfide, indole, motility) agar are both types of differential media used in microbiology to identify Enterobacteriaceae based on their ability to ferment sugars, produce hydrogen sulfide, and exhibit motility. Both media help differentiate between different bacterial species based on their specific metabolic activities and characteristics.


What is the best biochemical test to identify gram negative and H2S bacilli?

The best biochemical test to identify gram-negative and H2S-producing bacilli is the Triple Sugar Iron (TSI) test. This test uses a medium containing three different sugars (glucose, lactose, and sucrose) and ferrous sulfate to detect the production of hydrogen sulfide (H2S). Bacilli that ferment any of the sugars in the medium will produce acid, causing a color change in the medium, while the production of H2S will result in a black precipitate.


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Related questions

What is a Kliger's iron agar and SIM have in common?

Kliger's iron agar (KIA) and SIM (sulfide, indole, motility) agar are both types of differential media used in microbiology to identify Enterobacteriaceae based on their ability to ferment sugars, produce hydrogen sulfide, and exhibit motility. Both media help differentiate between different bacterial species based on their specific metabolic activities and characteristics.


What is the best biochemical test to identify gram negative and H2S bacilli?

The best biochemical test to identify gram-negative and H2S-producing bacilli is the Triple Sugar Iron (TSI) test. This test uses a medium containing three different sugars (glucose, lactose, and sucrose) and ferrous sulfate to detect the production of hydrogen sulfide (H2S). Bacilli that ferment any of the sugars in the medium will produce acid, causing a color change in the medium, while the production of H2S will result in a black precipitate.


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