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∙ 12y ago
High-performance liquid chromatography (HPLC) works by pumping a liquid sample through a column packed with tiny particles. These particles have different affinities for the components of the sample, causing them to separate as they pass through the column. The separated components are then detected by a detector, which produces a chromatogram. HPLC is commonly used in analytical chemistry to separate and quantify compounds in a mixture.
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What is the chromatography?
High Performance Liquid Chromatography.High Performance/Pressure Liquid Chromatography
What are the different types of chromatography?
The main types of chromatography include gas chromatography (GC), liquid chromatography (LC), thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC). Each type of chromatography separates compounds based on their differing affinities for a mobile phase and a stationary phase.
What is difference between high pressure liquid chromatography and high performance lequid chromatography?
High pressure liquid chromatography (HPLC) and high performance liquid chromatography (HPLC) are often used interchangeably. HPLC refers to modern liquid chromatography systems with high resolution and efficiency, while high pressure liquid chromatography specifically highlights the use of higher pressures in the system to improve separation and speed. Both terms generally refer to the same chromatographic technique.
How many types of Chromatography are there?
There are four main types of chromatography: gas chromatography (GC), liquid chromatography (LC), thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC). Each type of chromatography has specific applications and uses in separating and analyzing chemical compounds.
How is paper chromatography used in everyday life?
it can be used in everyday life by liquid chromatography, gas chromatography, thin-layer chromatographyand paper chromatography.
What is the chromatography?
High Performance Liquid Chromatography.High Performance/Pressure Liquid Chromatography
What has the author N A Parris written?
N. A. Parris has written: 'Instrumental liquid chromatography' -- subject(s): High performance liquid chromatography, Liquid chromatography
What are the different types of chromatography?
The main types of chromatography include gas chromatography (GC), liquid chromatography (LC), thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC). Each type of chromatography separates compounds based on their differing affinities for a mobile phase and a stationary phase.
What has the author Raymond P W Scott written?
Raymond P. W. Scott has written: 'Microbore Columns F L12' 'Contemporary liquid chromatography' -- subject(s): Liquid chromatography 'Liquid chromatography detectors' -- subject(s): Chromatographic detectors, Liquid chromatography 'Liquid chromatography for the analyst' -- subject(s): Liquid chromatography 'Chromatographic detectors' -- subject(s): Chromatographic detectors 'Techniques and practice of chromatography' -- subject(s): Chromatographic analysis
What are 3 types of chromatography?
1. thin -layer chromatography 2. gas chromatography 3. liquid chromatography
What is MPLC Chromatography?
MPLC stands for Medium-Pressure Liquid Chromatography, which is a chromatographic technique used for separating and purifying compounds based on their interactions with a stationary phase as they pass through a column under medium pressure. It is a versatile and efficient method commonly used in the purification of natural products, peptides, and other organic compounds.
What has the author R J Hamilton written?
R. J. Hamilton has written: 'Introduction to high performance liquid chromatography' -- subject(s): High performance liquid chromatography, Liquid chromatography
What has the author D Patel written?
D. Patel has written: 'Liquid chromatography' -- subject(s): Chromatography, Liquid, Laboratory manuals, Liquid chromatography, Handbooks 'Separating Cells (The Basics: from Background to Bench)'
How is paper chromatography used in everyday life?
it can be used in everyday life by liquid chromatography, gas chromatography, thin-layer chromatographyand paper chromatography.
What is the difference between partition chromatography and adsorption chromatography?
The partition chromatography involves separation between liquids while adsoption chromatography involves solid and liquid separations. Answer: Partition Chromatography is a liquid liquid extraction which involves two solvents while adsorption chromatography is a liquid solid extraction which involves a solid stationary phase & a liquid mobile phase.
What are some possible alternatives for paper chromatography?
Some possible alternatives for paper chromatography include thin-layer chromatography (TLC), gas chromatography, high-performance liquid chromatography (HPLC), and capillary electrophoresis. These techniques offer different separation mechanisms and have varying applications depending on the analyte and desired outcome.
What has the author Veronika Meyer written?
Veronika Meyer has written: 'Practical high-performance liquid chromatography' -- subject(s): High performance liquid chromatography 'Pitfalls and errors of HPLC in pictures' -- subject(s): High performance liquid chromatography
