Methanol breaks down the structure of the beet membrane which in turn release the pigment, betcyanin, the pigment found in beet cell vacuoles that give beets their characteristic colour, and releases it. The more intense the colour you see, the greater damage that had been done on the membrane
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Ethanol can disrupt cell wall structure by weakening the bonds between cellulose fibers, leading to decreased cell wall integrity and potentially causing cell damage or death. Ethanol can also affect enzyme activity involved in cell wall synthesis, disrupting the normal function and maintenance of the cell wall.
Acetone is an excellent organic solvent and in fact is soluble to some extent in water and lipid solutions. It will dissolve lipids in cell membranes and permeabilize them
The crystal violet stain in Gram-positive bacteria forms a complex with the peptidoglycan layer in the cell wall, making it less permeable to decolorizer like ethanol. In Gram-negative bacteria, the outer membrane is disrupted by ethanol, allowing the crystal violet to be washed out during the decolorization step.
Cell Wall
Cell Wall
The cell wall surrounds plant cells.
the cell wall, or it could also be the cell membrane, the cell wall is outside the cell membrane