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firs you mist know the polarity for sample, wen the sample polar you can use "RP" column like C18 or C8 ( C18 first in pharmaceutical) . wen sample non polar use "NP" column like silica or CN Column.

after that you can change the column in same packing to solve tailing, retention time, Resolution..... or any problem by change column length, particle size or carbon loud

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