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Enzyme-substrate complex (or ES complex) is the key to understand the kinetic behavior of the enzymes. The ES complex represents just the starting point for the catalysis reaction.


The kinetic pattern of enzymes was led by Victor Henri in 1903. He proposed that an enzyme combines with its substrate molecule to form the ES complex as a necessary step in enzyme catalysis. This idea expanded into a general theory of enzyme action, particularly by Leonor Michaelis and Maud Menten in 1913, who postulated that the enzyme (E) first combines reversibly with its substrate to form an enzyme-substrate complex (ES )in a relatively fast reversible step. The ES complex then breaks down in a slower second step to yield the free enzyme and a product (P), according to the following equation:


E + S < > ES > E + P

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Q: How can you describe the enzyme-substrate complex?
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