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Does HEPES dissociate

Updated: 5/30/2024
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9y ago

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HEPES is a type of zwitterionic organic chemical buffering agent. It is able to dissociate and its dissociation decreases as the temperature decreases.

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9y ago
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1mo ago

No, HEPES (4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid) does not dissociate into ions in solution. It is a buffer commonly used in cell culture media and biochemical experiments to maintain a stable pH.

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Q: Does HEPES dissociate
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How do you prepare 50 mM of HEPES?

To prepare a 50mM solution of HEPES, you would need to weigh out the appropriate amount of HEPES powder using a balance, dissolve it in the desired volume of water taking into account the molecular weight of HEPES, and then adjust the final volume to achieve the desired concentration. Finally, you can use a pH meter or pH indicator strips to adjust the pH as needed.


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If you make up a solution of 100 mL of 0.1 M HEPES in the basic form what will be the pH?

The pH of a 0.1 M HEPES solution is around 7.5. HEPES is a zwitterionic compound that acts as a buffering agent in the range of pH 6.8-8.2 due to its pKa values. At pH 7.5, it is mostly in the basic form.


What is the most efficient way to make up a HEPES buffer at pH 8.5 What starting compounds and reagents will you use?

To make a HEPES buffer at pH 8.5, you can start with HEPES acid and sodium hydroxide (NaOH) as the reagents. First, dissolve the appropriate amount of HEPES acid in water, then titrate with NaOH solution to adjust the pH to 8.5. Use a pH meter for accurate pH measurement during the titration process.


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Strong bases fully dissociate in water to produce hydroxide ions (OH-) and the cation. Weak bases partially dissociate in water to produce some hydroxide ions and the conjugate acid.


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