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13y ago
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4mo ago

Shorter restriction fragments travel farther in gel electrophoresis because they can move through the gel more easily due to their smaller size. Longer fragments experience more resistance and tend to travel a shorter distance through the gel.

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Q: Do longer or shorter restriction fragments travel farther?
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What is the effect of the run time on the observed pattern of restriction fragments?

The run time of an electrophoresis gel affects the separation of restriction fragments by size. Longer run times allow smaller fragments to travel further from the well, resulting in better resolution and clearer separation of fragments. Shorter run times may lead to overlapping bands and decreased resolution.


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The size of the DNA fragments separated during electrophoresis is primarily determined by their molecular weight. Larger fragments will migrate more slowly through the gel matrix, resulting in longer migration distances compared to smaller fragments. Additionally, the electric field strength applied during electrophoresis can also affect the distance traveled by the DNA fragments.


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